Computationally validated SARS-CoV-2 CTL and HTL Multi-Patch vaccines, designed by reverse epitomics approach, show potential to cover large ethnically distributed human population worldwide.
dc.contributor.author | Srivastava, Sukrit | |
dc.contributor.author | Verma, Sonia | |
dc.contributor.author | Kamthania, Mohit | |
dc.contributor.author | Agarwal, Deepa | |
dc.contributor.author | Saxena, Ajay Kumar | |
dc.contributor.author | Kolbe, Michael | |
dc.contributor.author | Singh, Sarman | |
dc.contributor.author | Kotnis, Ashwin | |
dc.contributor.author | Rathi, Brijesh | |
dc.contributor.author | Nayar, Seema A | |
dc.contributor.author | Shin, Ho-Joon | |
dc.contributor.author | Vashisht, Kapil | |
dc.contributor.author | Pandey, Kailash C | |
dc.date.accessioned | 2020-12-01T15:57:52Z | |
dc.date.available | 2020-12-01T15:57:52Z | |
dc.date.issued | 2020-11-06 | |
dc.identifier.citation | J Biomol Struct Dyn. 2020 Nov 6:1-20. doi: 10.1080/07391102.2020.1838329. Epub ahead of print. | en_US |
dc.identifier.pmid | 33155524 | |
dc.identifier.doi | 10.1080/07391102.2020.1838329 | |
dc.identifier.uri | http://hdl.handle.net/10033/622626 | |
dc.description.abstract | The SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) is responsible for the COVID-19 outbreak. The highly contagious COVID-19 disease has spread to 216 countries in less than six months. Though several vaccine candidates are being claimed, an effective vaccine is yet to come. A novel reverse epitomics approach, 'overlapping-epitope-clusters-to-patches' method is utilized to identify the antigenic regions from the SARS-CoV-2 proteome. These antigenic regions are named as 'Ag-Patch or Ag-Patches', for Antigenic Patch or Patches. The identification of Ag-Patches is based on the clusters of overlapping epitopes rising from SARS-CoV-2 proteins. Further, we have utilized the identified Ag-Patches to design Multi-Patch Vaccines (MPVs), proposing a novel method for the vaccine design. The designed MPVs were analyzed for immunologically crucial parameters, physiochemical properties and cDNA constructs. We identified 73 CTL (Cytotoxic T-Lymphocyte) and 49 HTL (Helper T-Lymphocyte) novel Ag-Patches from the proteome of SARS-CoV-2. The identified Ag-Patches utilized to design MPVs cover 768 overlapping epitopes targeting 55 different HLA alleles leading to 99.98% of world human population coverage. The MPVs and Toll-Like Receptor ectodomain complex shows stable complex formation tendency. Further, the cDNA analysis favors high expression of the MPVs constructs in a human cell line. We identified highly immunogenic novel Ag-Patches from the entire proteome of SARS CoV-2 by a novel reverse epitomics approach and utilized them to design MPVs. We conclude that the novel MPVs could be a highly potential novel approach to combat SARS-CoV-2, with greater effectiveness, high specificity and large human population coverage worldwide. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Taylor & Francis | en_US |
dc.rights | Attribution-NonCommercial-ShareAlike 4.0 International | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | * |
dc.subject | Ag-Patch (antigenic patch) | en_US |
dc.subject | COVID-19 | en_US |
dc.subject | Coronavirus | en_US |
dc.subject | Multi-Epitope Vaccine | en_US |
dc.subject | Multi-Patch Vaccine | en_US |
dc.subject | Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) | en_US |
dc.subject | Toll-Like Receptor (TLR) | en_US |
dc.subject | epitope | en_US |
dc.subject | overlapping-epitope-clusters-to-patches | en_US |
dc.subject | reverse epitomics | en_US |
dc.title | Computationally validated SARS-CoV-2 CTL and HTL Multi-Patch vaccines, designed by reverse epitomics approach, show potential to cover large ethnically distributed human population worldwide. | en_US |
dc.type | Article | en_US |
dc.identifier.eissn | 1538-0254 | |
dc.contributor.department | CSSB, Centre for Structural Systembiologie, Notkestr.85, 22607 Hamburg. Germany. | en_US |
dc.identifier.journal | Journal of biomolecular structure & dynamics | en_US |
dc.source.beginpage | 1 | |
dc.source.endpage | 20 | |
refterms.dateFOA | 2020-12-01T15:57:52Z | |
dc.source.journaltitle | Journal of biomolecular structure & dynamics | |
dc.source.country | England |