Loss of Ena/VASP interferes with lamellipodium architecture, motility and integrin-dependent adhesion.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
MetadataShow full item record
AbstractCell migration entails networks and bundles of actin filaments termed lamellipodia and microspikes or filopodia, respectively, as well as focal adhesions, all of which recruit Ena/VASP family members hitherto thought to antagonize efficient cell motility. However, we find these proteins to act as positive regulators of migration in different murine cell lines. CRISPR/Cas9-mediated loss of Ena/VASP proteins reduced lamellipodial actin assembly and perturbed lamellipodial architecture, as evidenced by changed network geometry as well as reduction of filament length and number that was accompanied by abnormal Arp2/3 complex and heterodimeric capping protein accumulation. Loss of Ena/VASP function also abolished the formation of microspikes normally embedded in lamellipodia, but not of filopodia capable of emanating without lamellipodia. Ena/VASP-deficiency also impaired integrin-mediated adhesion accompanied by reduced traction forces exerted through these structures. Our data thus uncover novel Ena/VASP functions of these actin polymerases that are fully consistent with their promotion of cell migration.
CitationElife. 2020 May 11;9:e55351. doi: 10.7554/eLife.55351.
AffiliationHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.
PublishereLife Sciences Publications
The following license files are associated with this item:
- Creative Commons
- Antagonism between Ena/VASP proteins and actin filament capping regulates fibroblast motility.
- Authors: Bear JE, Svitkina TM, Krause M, Schafer DA, Loureiro JJ, Strasser GA, Maly IV, Chaga OY, Cooper JA, Borisy GG, Gertler FB
- Issue date: 2002 May 17
- Efficiency of lamellipodia protrusion is determined by the extent of cytosolic actin assembly.
- Authors: Dimchev G, Steffen A, Kage F, Dimchev V, Pernier J, Carlier MF, Rottner K
- Issue date: 2017 May 15
- Ena/VASP proteins have an anti-capping independent function in filopodia formation.
- Authors: Applewhite DA, Barzik M, Kojima S, Svitkina TM, Gertler FB, Borisy GG
- Issue date: 2007 Jul
- Capping protein is dispensable for polarized actin network growth and actin-based motility.
- Authors: Abou-Ghali M, Kusters R, Körber S, Manzi J, Faix J, Sykes C, Plastino J
- Issue date: 2020 Nov 6
- Ena/VASP proteins can regulate distinct modes of actin organization at cadherin-adhesive contacts.
- Authors: Scott JA, Shewan AM, den Elzen NR, Loureiro JJ, Gertler FB, Yap AS
- Issue date: 2006 Mar