Triple RNA-Seq Reveals Synergy in a Human Virus-Fungus Co-infection Model.
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Authors
Seelbinder, BastianWallstabe, Julia
Marischen, Lothar
Weiss, Esther
Wurster, Sebastian
Page, Lukas
Löffler, Claudia
Bussemer, Lydia
Schmitt, Anna-Lena
Wolf, Thomas
Linde, Jörg
Cicin-Sain, Luka
Becker, Jennifer
Kalinke, Ulrich
Vogel, Jörg
Panagiotou, Gianni
Einsele, Hermann
Westermann, Alexander J
Schäuble, Sascha
Loeffler, Juergen
Issue Date
2020-11-17
Metadata
Show full item recordAbstract
High-throughput RNA sequencing (RNA-seq) is routinely applied to study diverse biological processes; however, when performed separately on interacting organisms, systemic noise intrinsic to RNA extraction, library preparation, and sequencing hampers the identification of cross-species interaction nodes. Here, we develop triple RNA-seq to simultaneously detect transcriptomes of monocyte-derived dendritic cells (moDCs) infected with the frequently co-occurring pulmonary pathogens Aspergillus fumigatus and human cytomegalovirus (CMV). Comparing expression patterns after co-infection with those after single infections, our data reveal synergistic effects and mutual interferences between host responses to the two pathogens. For example, CMV attenuates the fungus-mediated activation of pro-inflammatory cytokines through NF-κB (nuclear factor κB) and NFAT (nuclear factor of activated T cells) cascades, while A. fumigatus impairs viral clearance by counteracting viral nucleic acid-induced activation of type I interferon signaling. Together, the analytical power of triple RNA-seq proposes molecular hubs in the differential moDC response to fungal/viral single infection or co-infection that contribute to our understanding of the etiology and, potentially, clearance of post-transplant infections.Citation
Cell Rep. 2020 Nov 17;33(7):108389. doi: 10.1016/j.celrep.2020.108389.Affiliation
HIRI, Helmholtz-Institut für RNA-basierte Infektionsforschung, Josef-Shneider Strasse 2, 97080 Würzburg, Germany.; HZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.Publisher
Elsevier (Cell Press)Journal
Cell reportsPubMed ID
33207195Type
ArticleLanguage
enEISSN
2211-1247ae974a485f413a2113503eed53cd6c53
10.1016/j.celrep.2020.108389
Scopus Count
The following license files are associated with this item:
- Creative Commons
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