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dc.contributor.authorSeelbinder, Bastian
dc.contributor.authorWallstabe, Julia
dc.contributor.authorMarischen, Lothar
dc.contributor.authorWeiss, Esther
dc.contributor.authorWurster, Sebastian
dc.contributor.authorPage, Lukas
dc.contributor.authorLöffler, Claudia
dc.contributor.authorBussemer, Lydia
dc.contributor.authorSchmitt, Anna-Lena
dc.contributor.authorWolf, Thomas
dc.contributor.authorLinde, Jörg
dc.contributor.authorCicin-Sain, Luka
dc.contributor.authorBecker, Jennifer
dc.contributor.authorKalinke, Ulrich
dc.contributor.authorVogel, Jörg
dc.contributor.authorPanagiotou, Gianni
dc.contributor.authorEinsele, Hermann
dc.contributor.authorWestermann, Alexander J
dc.contributor.authorSchäuble, Sascha
dc.contributor.authorLoeffler, Juergen
dc.date.accessioned2020-12-08T14:54:28Z
dc.date.available2020-12-08T14:54:28Z
dc.date.issued2020-11-17
dc.identifier.citationCell Rep. 2020 Nov 17;33(7):108389. doi: 10.1016/j.celrep.2020.108389.en_US
dc.identifier.pmid33207195
dc.identifier.doi10.1016/j.celrep.2020.108389
dc.identifier.urihttp://hdl.handle.net/10033/622636
dc.description.abstractHigh-throughput RNA sequencing (RNA-seq) is routinely applied to study diverse biological processes; however, when performed separately on interacting organisms, systemic noise intrinsic to RNA extraction, library preparation, and sequencing hampers the identification of cross-species interaction nodes. Here, we develop triple RNA-seq to simultaneously detect transcriptomes of monocyte-derived dendritic cells (moDCs) infected with the frequently co-occurring pulmonary pathogens Aspergillus fumigatus and human cytomegalovirus (CMV). Comparing expression patterns after co-infection with those after single infections, our data reveal synergistic effects and mutual interferences between host responses to the two pathogens. For example, CMV attenuates the fungus-mediated activation of pro-inflammatory cytokines through NF-κB (nuclear factor κB) and NFAT (nuclear factor of activated T cells) cascades, while A. fumigatus impairs viral clearance by counteracting viral nucleic acid-induced activation of type I interferon signaling. Together, the analytical power of triple RNA-seq proposes molecular hubs in the differential moDC response to fungal/viral single infection or co-infection that contribute to our understanding of the etiology and, potentially, clearance of post-transplant infections.en_US
dc.language.isoenen_US
dc.publisherElsevier (Cell Press)en_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectAspergillusen_US
dc.subjectcross-species interactionen_US
dc.subjectcytomegalovirusen_US
dc.subjectdendritic cellsen_US
dc.subjecthost responseen_US
dc.subjectpulmonary infectionsen_US
dc.subjectsynergyen_US
dc.subjecttranscriptional networksen_US
dc.subjecttriple RNA-seqen_US
dc.titleTriple RNA-Seq Reveals Synergy in a Human Virus-Fungus Co-infection Model.en_US
dc.typeArticleen_US
dc.identifier.eissn2211-1247
dc.contributor.departmentHIRI, Helmholtz-Institut für RNA-basierte Infektionsforschung, Josef-Shneider Strasse 2, 97080 Würzburg, Germany.; HZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalCell reportsen_US
dc.source.volume33
dc.source.issue7
dc.source.beginpage108389
dc.source.endpage
refterms.dateFOA2020-12-08T14:54:29Z
dc.source.journaltitleCell reports
dc.source.countryUnited States


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International