Dudomycins: New Secondary Metabolites Produced After Heterologous Expression of an Nrps Cluster from ssp. Nrrl B-24108.
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Authors
Lasch, ConstanzeStierhof, Marc
Estévez, Marta Rodríguez
Myronovskyi, Maksym
Zapp, Josef
Luzhetskyy, Andriy
Issue Date
2020-11-16
Metadata
Show full item recordAbstract
Since the 1950s, natural products of bacterial origin were systematically developed to be used as drugs with a wide range of medical applications. The available treatment options for many diseases are still not satisfying, wherefore, the discovery of new structures has not lost any of its importance. Beyond the great variety of already isolated and characterized metabolites, Streptomycetes still harbor uninvestigated gene clusters whose products can be accessed using heterologous expression in host organisms. This works presents the discovery of a set of structurally novel secondary metabolites, dudomycins A to D, through the expression of a cryptic NRPS cluster from Streptomyces albus ssp. Chlorinus NRRL B-24108 in the heterologous host strain Streptomyces albus Del14. A minimal set of genes, required for the production of dudomycins, was defined through gene inactivation experiments. This paper also proposes a model for dudomycin biosynthesis.Citation
Microorganisms. 2020 Nov 16;8(11):1800. doi: 10.3390/microorganisms8111800.Affiliation
HIPS, Helmholtz-Institut für Pharmazeutische Forschung Saarland, Universitätscampus E8.1 66123 Saarbrücken, Germany.Publisher
MDPIJournal
MicroorganismsPubMed ID
33207845Type
ArticleLanguage
enISSN
2076-2607ae974a485f413a2113503eed53cd6c53
10.3390/microorganisms8111800
Scopus Count
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- Creative Commons
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