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dc.contributor.authorBader, Chantal D
dc.contributor.authorNeuber, Markus
dc.contributor.authorPanter, Fabian
dc.contributor.authorKrug, Daniel
dc.contributor.authorMüller, Rolf
dc.date.accessioned2021-01-07T13:39:08Z
dc.date.available2021-01-07T13:39:08Z
dc.date.issued2020-11-10
dc.identifier.citationAnal Chem. 2020 Dec 1;92(23):15403-15411. doi: 10.1021/acs.analchem.0c02995. Epub 2020 Nov 10.en_US
dc.identifier.pmid33171050
dc.identifier.doi10.1021/acs.analchem.0c02995
dc.identifier.urihttp://hdl.handle.net/10033/622667
dc.description.abstractSupercritical fluid extraction (SFE) is widely used for the isolation of natural products from plants, but its application in efforts to identify structurally and physicochemically often dissimilar microbial natural products is limited to date. In this study, we evaluated the impact of SFE on the extractability of myxobacterial secondary metabolites, aiming to improve the prospects of discovering novel natural products. We investigated the influence of different co-solvents on the extraction efficiency of secondary metabolites from three myxobacterial strains and the antimicrobial activity profiles of the corresponding extracts. For each known secondary metabolite, we found extraction conditions using SFE leading to superior yields in the extracts compared to conventional solvent extraction. Compounds with a logP higher than 3 showed the best extraction efficiency using 20% EtOAc as a co-solvent, whereas compounds with logP values lower than 3 were better extractable using more polar co-solvents such as MeOH. Extracts generated with SFE showed increased antimicrobial activities including the presence of activities not explained by known myxobacterial secondary metabolites, highlighting the advantage of SFE for bioactivity-guided isolation. Moreover, non-targeted metabolomics analysis revealed a group of chlorinated metabolites produced by the well-studied model myxobacterium Myxococcus xanthus DK1622, which were not accessible previously due to their low concentration in conventional extracts. The enriched SF extracts were used for isolation and subsequent structure elucidation of chloroxanthic acid A as the founding member of a novel secondary metabolite family. Our findings encourage the increased utilization of SFE as a part of future screening workflows of microbial natural products.en_US
dc.language.isoenen_US
dc.publisherAmerican Chemical Societyen_US
dc.titleSupercritical Fluid Extraction Enhances Discovery of Secondary Metabolites from Myxobacteria.en_US
dc.typeArticleen_US
dc.identifier.eissn1520-6882
dc.contributor.departmentHIPS, Helmholtz-Institut für Pharmazeutische Forschung Saarland, Universitätscampus E8.1 66123 Saarbrücken, Germany.en_US
dc.identifier.journalAnalytical chemistryen_US
dc.source.volume92
dc.source.issue23
dc.source.beginpage15403
dc.source.endpage15411
dc.source.journaltitleAnalytical chemistry
dc.source.countryUnited States


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