Simultaneous Presence of Bacteriochlorophyll and Xanthorhodopsin Genes in a Freshwater Bacterium.
Average rating
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Star rating
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Authors
Kopejtka, KarelTomasch, Jürgen
Zeng, Yonghui
Selyanin, Vadim
Dachev, Marko
Piwosz, Kasia
Tichý, Martin
Bína, David
Gardian, Zdenko
Bunk, Boyke
Brinkmann, Henner
Geffers, Robert
Sommaruga, Ruben
Koblížek, Michal
Issue Date
2020-12-22
Metadata
Show full item recordAbstract
Photoheterotrophic bacteria represent an important part of aquatic microbial communities. There exist two fundamentally different light-harvesting systems: bacteriochlorophyll-containing reaction centers or rhodopsins. Here, we report a photoheterotrophic Sphingomonas strain isolated from an oligotrophic lake, which contains complete sets of genes for both rhodopsin-based and bacteriochlorophyll-based phototrophy. Interestingly, the identified genes were not expressed when cultured in liquid organic media. Using reverse transcription quantitative PCR (RT-qPCR), RNA sequencing, and bacteriochlorophyll a quantification, we document that bacteriochlorophyll synthesis was repressed by high concentrations of glucose or galactose in the medium. Coactivation of photosynthesis genes together with genes for TonB-dependent transporters suggests the utilization of light energy for nutrient import. The photosynthetic units were formed by ring-shaped light-harvesting complex 1 and reaction centers with bacteriochlorophyll a and spirilloxanthin as the main light-harvesting pigments. The identified rhodopsin gene belonged to the xanthorhodopsin family, but it lacks salinixanthin antenna. In contrast to bacteriochlorophyll, the expression of xanthorhodopsin remained minimal under all experimental conditions tested. Since the gene was found in the same operon as a histidine kinase, we propose that it might serve as a light sensor. Our results document that photoheterotrophic Sphingomonas bacteria use the energy of light under carbon-limited conditions, while under carbon-replete conditions, they cover all their metabolic needs through oxidative phosphorylation.IMPORTANCE Phototrophic organisms are key components of many natural environments. There exist two main phototrophic groups: species that collect light energy using various kinds of (bacterio)chlorophylls and species that utilize rhodopsins. Here, we present a freshwater bacterium Sphingomonas sp. strain AAP5 which contains genes for both light-harvesting systems. We show that bacteriochlorophyll-based reaction centers are repressed by light and/or glucose. On the other hand, the rhodopsin gene was not expressed significantly under any of the experimental conditions. This may indicate that rhodopsin in Sphingomonas may have other functions not linked to bioenergetics.Citation
mSystems. 2020 Dec 22;5(6):e01044-20. doi: 10.1128/mSystems.01044-20.Affiliation
HZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.Publisher
ASMJournal
mSystemsPubMed ID
33361324Type
ArticleLanguage
enISSN
2379-5077ae974a485f413a2113503eed53cd6c53
10.1128/mSystems.01044-20
Scopus Count
The following license files are associated with this item:
- Creative Commons
Related articles
- A bacterium from a mountain lake harvests light using both proton-pumping xanthorhodopsins and bacteriochlorophyll-based photosystems.
- Authors: Kopejtka K, Tomasch J, Kaftan D, Gardiner AT, Bína D, Gardian Z, Bellas C, Dröge A, Geffers R, Sommaruga R, Koblížek M
- Issue date: 2022 Dec 13
- Potential Rhodopsin- and Bacteriochlorophyll-Based Dual Phototrophy in a High Arctic Glacier.
- Authors: Zeng Y, Chen X, Madsen AM, Zervas A, Nielsen TK, Andrei AS, Lund-Hansen LC, Liu Y, Hansen LH
- Issue date: 2020 Nov 24
- Characterization of the Aerobic Anoxygenic Phototrophic Bacterium Sphingomonas sp. AAP5.
- Authors: Kopejtka K, Zeng Y, Kaftan D, Selyanin V, Gardian Z, Tomasch J, Sommaruga R, Koblížek M
- Issue date: 2021 Apr 6
- Genomic Analysis of the Evolution of Phototrophy among Haloalkaliphilic Rhodobacterales.
- Authors: Kopejtka K, Tomasch J, Zeng Y, Tichý M, Sorokin DY, Koblížek M
- Issue date: 2017 Jul 1
- Emended description of the genus Tabrizicola and the species Tabrizicola aquatica as aerobic anoxygenic phototrophic bacteria.
- Authors: Tarhriz V, Hirose S, Fukushima SI, Hejazi MA, Imhoff JF, Thiel V, Hejazi MS
- Issue date: 2019 Aug