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dc.contributor.authorQi, Cancan
dc.contributor.authorVonk, Judith M
dc.contributor.authorvan der Plaat, Diana A
dc.contributor.authorNieuwenhuis, Maartje A E
dc.contributor.authorDijk, F Nicole
dc.contributor.authorAïssi, Dylan
dc.contributor.authorSiroux, Valérie
dc.contributor.authorBoezen, H Marike
dc.contributor.authorXu, Cheng-Jian
dc.contributor.authorKoppelman, Gerard H
dc.date.accessioned2021-01-08T15:39:02Z
dc.date.available2021-01-08T15:39:02Z
dc.date.issued2020-12-11
dc.identifier.citationClin Transl Allergy. 2020 Dec 11;10(1):60. doi: 10.1186/s13601-020-00365-4.en_US
dc.identifier.issn2045-7022
dc.identifier.pmid33303027
dc.identifier.doi10.1186/s13601-020-00365-4
dc.identifier.urihttp://hdl.handle.net/10033/622670
dc.description.abstractBackground: Asthma is a chronic respiratory disease which is not curable, yet some patients experience spontaneous remission. We hypothesized that epigenetic mechanisms may be involved in asthma remission. Methods: Clinical remission (ClinR) was defined as the absence of asthma symptoms and medication for at least 12 months, and complete remission (ComR) was defined as ClinR with normal lung function and absence of airway hyperresponsiveness. We analyzed differential DNA methylation of ClinR and ComR comparing to persistent asthma (PersA) in whole blood samples (n = 72) and nasal brushing samples (n = 97) in a longitudinal cohort of well characterized asthma patients. Significant findings of whole blood DNA methylation were tested for replication in two independent cohorts, Lifelines and Epidemiological study on the Genetics and Environment of Asthma (EGEA). Results: We identified differentially methylated CpG sites associated with ClinR (7 CpG sites) and ComR (129 CpG sites) in whole blood. One CpG (cg13378519, Chr1) associated with ClinR was replicated and annotated to PEX11 (Peroxisomal Biogenesis Factor 11 Beta). The whole blood DNA methylation levels of this CpG were also different between ClinR and healthy subjects. One ComR-associated CpG (cg24788483, Chr10) that annotated to TCF7L2 (Transcription Factor 7 Like 2) was replicated and associated with expression of TCF7L2 gene. One out of seven ClinR-associated CpG sites and 8 out of 129 ComR-associated CpG sites identified from whole blood samples showed nominal significance (P < 0.05) and the same direction of effect in nasal brushes. Conclusion: We identified DNA methylation markers possibly associated with clinical and complete asthma remission in nasal brushes and whole blood, and two CpG sites identified from whole blood can be replicated in independent cohorts and may play a role in peroxisome proliferation and Wnt signaling pathway.en_US
dc.language.isoenen_US
dc.publisherBMCen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectAsthma remissionen_US
dc.subjectDNA methylationen_US
dc.subjectNasal brushesen_US
dc.subjectWhole blooden_US
dc.titleEpigenome-wide association study identifies DNA methylation markers for asthma remission in whole blood and nasal epithelium.en_US
dc.typeArticleen_US
dc.contributor.departmentCiiM, Zentrum für individualisierte Infektionsmedizin, Feodor-Lynen-Str.7, 30625 Hannover.en_US
dc.identifier.journalClinical and translational allergyen_US
dc.source.volume10
dc.source.issue1
dc.source.beginpage60
dc.source.endpage
refterms.dateFOA2021-01-08T15:39:02Z
dc.source.journaltitleClinical and translational allergy
dc.source.countryEngland


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International