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dc.contributor.authorMichaux, Charlotte
dc.contributor.authorHansen, Elisabeth E
dc.contributor.authorJenniches, Laura
dc.contributor.authorGerovac, Milan
dc.contributor.authorBarquist, Lars
dc.contributor.authorVogel, Jörg
dc.date.accessioned2021-01-11T14:34:31Z
dc.date.available2021-01-11T14:34:31Z
dc.date.issued2020-11-25
dc.identifier.pmid33324581
dc.identifier.doi10.3389/fcimb.2020.600325
dc.identifier.urihttp://hdl.handle.net/10033/622671
dc.description.abstractEnterococcus faecalis and faecium are two major representative clinical strains of the Enterococcus genus and are sadly notorious to be part of the top agents responsible for nosocomial infections. Despite their critical implication in worldwide public healthcare, essential and available resources such as deep transcriptome annotations remain poor, which also limits our understanding of post-transcriptional control small regulatory RNA (sRNA) functions in these bacteria. Here, using the dRNA-seq technique in combination with ANNOgesic analysis, we successfully mapped and annotated transcription start sites (TSS) of both E. faecalis V583 and E. faecium AUS0004 at single nucleotide resolution. Analyzing bacteria in late exponential phase, we capture ~40% (E. faecalis) and 43% (E. faecium) of the annotated protein-coding genes, determine 5' and 3' UTR (untranslated region) length, and detect instances of leaderless mRNAs. The transcriptome maps revealed sRNA candidates in both bacteria, some found in previous studies and new ones. Expression of candidate sRNAs is being confirmed under biologically relevant environmental conditions. This comprehensive global TSS mapping atlas provides a valuable resource for RNA biology and gene expression analysis in the Enterococci. It can be accessed online at www.helmholtz-hiri.de/en/datasets/enterococcus through an instance of the genomic viewer JBrowse.en_US
dc.language.isoenen_US
dc.publisherFrontiersen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectGram-positive bacteriaen_US
dc.subjectRNA-seqen_US
dc.subjectpost-transcriptional regulationen_US
dc.subjectsRNA atlasen_US
dc.subjecttranscription start sitesen_US
dc.titleSingle-Nucleotide RNA Maps for the Two Major Nosocomial Pathogens Enterococcus faecalis and Enterococcus faeciumen_US
dc.typeArticleen_US
dc.identifier.eissn2235-2988
dc.contributor.departmentHIRI, Helmholtz-Institut für RNA-basierte Infektionsforschung, Josef-Shneider Strasse 2, 97080 Würzburg, Germany.en_US
dc.identifier.journalFrontiers in cellular and infection microbiologyen_US
dc.source.volume10
dc.source.beginpage600325
dc.source.endpage
refterms.dateFOA2021-01-11T14:34:32Z
atmire.accessrights
dc.source.journaltitleFrontiers in cellular and infection microbiology
dc.source.countrySwitzerland


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International