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dc.contributor.authorVenturini, Elisa
dc.contributor.authorSvensson, Sarah L
dc.contributor.authorMaaß, Sandra
dc.contributor.authorGelhausen, Rick
dc.contributor.authorEggenhofer, Florian
dc.contributor.authorLi, Lei
dc.contributor.authorCain, Amy K
dc.contributor.authorParkhill, Julian
dc.contributor.authorBecher, Dörte
dc.contributor.authorBackofen, Rolf
dc.contributor.authorBarquist, Lars
dc.contributor.authorSharma, Cynthia M
dc.contributor.authorWestermann, Alexander J
dc.contributor.authorVogel, Jörg
dc.date.accessioned2021-01-21T13:41:16Z
dc.date.available2021-01-21T13:41:16Z
dc.date.issued2020-10-17
dc.identifier.citationMicroLife(2020) .advance publication; DOI: 10.1093/femsml/uqaa002 .en_US
dc.identifier.doi10.1093/femsml/uqaa002
dc.identifier.urihttp://hdl.handle.net/10033/622691
dc.description.abstractSmall proteins are an emerging class of gene products with diverse roles in bacterial physiology. However, a full understanding of their importance has been hampered by insufficient genome annotations and a lack of comprehensive characterization in microbes other than Escherichia coli. We have taken an integrative approach to accelerate the discovery of small proteins and their putative virulence-associated functions in Salmonella Typhimurium. We merged the annotated small proteome of Salmonella with new small proteins predicted with in silico and experimental approaches. We then exploited existing and newly generated global datasets that provide information on small open reading frame expression during infection of epithelial cells (dual RNA-seq), contribution to bacterial fitness inside macrophages (Transposon-directed insertion sequencing), and potential engagement in molecular interactions (Grad-seq). This integrative approach suggested a new role for the small protein MgrB beyond its known function in regulating PhoQ. We demonstrate a virulence and motility defect of a Salmonella ΔmgrB mutant and reveal an effect of MgrB in regulating the Salmonella transcriptome and proteome under infection-relevant conditions. Our study highlights the power of interpreting available ‘omics’ datasets with a focus on small proteins, and may serve as a blueprint for a data integration-based survey of small proteins in diverse bacteria.en_US
dc.description.sponsorshipAustralian Research Councilen_US
dc.language.isoenen_US
dc.publisherOxford University Press (OUP)en_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleA global data-driven census of Salmonella small proteins and their potential functions in bacterial virulenceen_US
dc.typeArticleen_US
dc.identifier.eissn2633-6693
dc.contributor.departmentHIRI, Helmholtz-Institut für RNA-basierte Infektionsforschung, Josef-Shneider Strasse 2, 97080 Würzburg, Germany.en_US
dc.identifier.journalMicroLifeen_US
dc.source.volume1
dc.source.issue1
refterms.dateFOA2021-01-21T13:41:17Z
dc.source.journaltitlemicroLife


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International