Show simple item record

dc.contributor.authorLi, Zhaopeng
dc.contributor.authorRinas, Ursula
dc.date.accessioned2021-01-27T13:16:12Z
dc.date.available2021-01-27T13:16:12Z
dc.date.issued2020-04-06
dc.identifier.citationMicrob Cell Fact. 2020 Apr 6;19(1):83. doi: 10.1186/s12934-020-01343-y.en_US
dc.identifier.pmid32252765
dc.identifier.doi10.1186/s12934-020-01343-y
dc.identifier.urihttp://hdl.handle.net/10033/622704
dc.description.abstractBackground: Recombinant protein production can be stressful to the host organism. The extent of stress is determined by the specific properties of the recombinant transcript and protein, by the rates of transcription and translation, and by the environmental conditions encountered during the production process. Results: The impact of the transcription of the T7-promoter controlled genes encoding human basic fibroblast growth factor (hFGF-2) and green fluorescent protein (GFP) as well as the translation into the recombinant protein on the growth properties of the production host E. coli BL21(DE3) were investigated. This was done by using expression vectors where the promoter region or the ribosome binding site(s) or both were removed. It is shown that already transcription without protein translation imposes a metabolic burden on the host cell. Translation of the transcript into large amounts of a properly folded protein does not show any effect on cell growth in the best case, e.g. high-level production of GFP in Luria-Bertani medium. However, translation appears to contribute to the metabolic burden if it is connected to protein folding associated problems, e.g. inclusion body formation. Conclusion: The so-called metabolic burden of recombinant protein production is mainly attributed to transcription but can be enhanced through translation and those processes following translation (e.g. protein folding and degradation, heat-shock responses).en_US
dc.language.isoenen_US
dc.publisherBMC (part of Springer)en_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectEscherichia colien_US
dc.subjectMetabolic burdenen_US
dc.subjectRecombinant protein productionen_US
dc.subjectTranscriptional burdenen_US
dc.titleRecombinant protein production associated growth inhibition results mainly from transcription and not from translation.en_US
dc.typeArticleen_US
dc.identifier.eissn1475-2859
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalMicrobial cell factoriesen_US
dc.source.volume19
dc.source.issue1
dc.source.beginpage83
dc.source.endpage
refterms.dateFOA2021-01-27T13:16:13Z
dc.source.journaltitleMicrobial cell factories
dc.source.countryEngland


Files in this item

Thumbnail
Name:
Li and Rinas_b.pdf
Size:
4.132Mb
Format:
PDF
Description:
Open Access publication

This item appears in the following Collection(s)

Show simple item record

Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International