Show simple item record

dc.contributor.authorKorf, Imke H E
dc.contributor.authorKittler, Sophie
dc.contributor.authorBierbrodt, Anna
dc.contributor.authorMengden, Ruth
dc.contributor.authorRohde, Christine
dc.contributor.authorRohde, Manfred
dc.contributor.authorKroj, Andrea
dc.contributor.authorLehnherr, Tatiana
dc.contributor.authorFruth, Angelika
dc.contributor.authorFlieger, Antje
dc.contributor.authorLehnherr, Hansjörg
dc.contributor.authorWittmann, Johannes
dc.date.accessioned2021-02-02T13:01:31Z
dc.date.available2021-02-02T13:01:31Z
dc.date.issued2020-12-19
dc.identifier.citationViruses. 2020 Dec 19;12(12):1470. doi: 10.3390/v12121470.en_US
dc.identifier.pmid33352791
dc.identifier.doi10.3390/v12121470
dc.identifier.urihttp://hdl.handle.net/10033/622712
dc.description.abstractWorldwide, poultry industry suffers from infections caused by avian pathogenic Escherichia coli. Therapeutic failure due to resistant bacteria is of increasing concern and poses a threat to human and animal health. This causes a high demand to find alternatives to fight bacterial infections in animal farming. Bacteriophages are being especially considered for the control of multi-drug resistant bacteria due to their high specificity and lack of serious side effects. Therefore, the study aimed on characterizing phages and composing a phage cocktail suitable for the prevention of infections with E. coli. Six phages were isolated or selected from our collections and characterized individually and in combination with regard to host range, stability, reproduction, and efficacy in vitro. The cocktail consisting of six phages was able to inhibit formation of biofilms by some E. coli strains but not by all. Phage-resistant variants arose when bacterial cells were challenged with a single phage but not when challenged by a combination of four or six phages. Resistant variants arising showed changes in carbon metabolism and/or motility. Genomic comparison of wild type and phage-resistant mutant E28.G28R3 revealed a deletion of several genes putatively involved in phage adsorption and infection.en_US
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectAPECen_US
dc.subjectE. colien_US
dc.subjectbacteriophagesen_US
dc.subjectbiofilmen_US
dc.subjectcolibacillosisen_US
dc.subjectmultidrug-resistant bacteriaen_US
dc.subjectphage cocktailen_US
dc.subjectphage-resistant variantsen_US
dc.titleIn Vitro Evaluation of a Phage Cocktail Controlling Infections with Escherichia coli.en_US
dc.typeArticleen_US
dc.identifier.eissn1999-4915
dc.contributor.departmentTWINCORE, Zentrum für experimentelle und klinische Infektionsforschung GmbH,Feodor-Lynen Str. 7, 30625 Hannover, Germany.en_US
dc.identifier.journalVirusesen_US
dc.source.volume12
dc.source.issue12
dc.source.journaltitleViruses
dc.source.countrySwitzerland


Files in this item

Thumbnail
Name:
Korf et al.pdf
Size:
2.660Mb
Format:
PDF
Description:
delayed Open Access publication

This item appears in the following Collection(s)

Show simple item record

Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International