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dc.contributor.authorNakagawa, Rinako
dc.contributor.authorToboso-Navasa, Amparo
dc.contributor.authorSchips, Marta
dc.contributor.authorYoung, George
dc.contributor.authorBhaw-Rosun, Leena
dc.contributor.authorLlorian-Sopena, Miriam
dc.contributor.authorChakravarty, Probir
dc.contributor.authorSesay, Abdul Karim
dc.contributor.authorKassiotis, George
dc.contributor.authorMeyer-Hermann, Michael
dc.contributor.authorCalado, Dinis Pedro
dc.identifier.citationProc Natl Acad Sci U S A. 2021 Jan 12;118(2):e2016425118. doi: 10.1073/pnas.2016425118.en_US
dc.description.abstractAffinity maturation depends on how efficiently germinal centers (GCs) positively select B cells in the light zone (LZ). Positively selected GC B cells recirculate between LZs and dark zones (DZs) and ultimately differentiate into plasmablasts (PBs) and memory B cells (MBCs). Current understanding of the GC reaction presumes that cMyc-dependent positive selection of LZ B cells is a competitive affinity-dependent process; however, this cannot explain the production of GC-derived lower-affinity MBCs or retention of GC B cells with varied affinities. Here, by combining single-cell/bulk RNA sequencing and flow cytometry, we identified and characterized temporally and functionally distinct positively selected cMyc+ GC B cell subpopulations. cMyc+ LZ B cell subpopulations enriched with either higher- or lower-affinity cells diverged soon after permissive positive selection. The former subpopulation contained PB precursors, whereas the latter comprised less proliferative MBC precursors and future DZ entrants. The overall affinity of future DZ entrants was enhanced in the LZ through preferential proliferation of higher-affinity cells. Concurrently, lower-affinity cells were retained in GCs and protected from apoptosis. These findings redefine positive selection as a dynamic process generating three distinct B cell fates and elucidate how positive selection ensures clonal diversity for broad protection.en_US
dc.publisherNational Academy of Sciencesen_US
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.subjectGC B cellsen_US
dc.subjectaffinity maturationen_US
dc.subjectclonal diversityen_US
dc.subjectmemory B cellsen_US
dc.subjectpositive selectionen_US
dc.titlePermissive selection followed by affinity-based proliferation of GC light zone B cells dictates cell fate and ensures clonal breadth.en_US
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalProceedings of the National Academy of Sciences of the United States of Americaen_US
dc.source.journaltitleProceedings of the National Academy of Sciences of the United States of America
dc.source.countryUnited Kingdom
dc.source.countryUnited States

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Attribution-NonCommercial-NoDerivatives 4.0 International
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