Genetic determinants of Pseudomonas aeruginosa fitness during biofilm growth.
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Authors
Schinner, SilviaEngelhardt, Florian
Preusse, Matthias
Thöming, Janne Gesine
Tomasch, Jürgen
Häussler, Susanne
Issue Date
2020-04-02
Metadata
Show full item recordAbstract
Pseudomonas aeruginosa is an environmental bacterium and an opportunistic human pathogen. It is also a well-established model organism to study bacterial adaptation to stressful conditions, such as those encountered during an infection process in the human host. Advancing knowledge on P. aeruginosa adaptation to biofilm growth conditions is bound to reveal novel strategies and targets for the treatment of chronic biofilm-associated infections. Here, we generated transposon insertion libraries in three P. aeruginosa strain backgrounds and determined the relative frequency of each insertion following biofilm growth using transposon sequencing. We demonstrate that in general the SOS response, several tRNA modifying enzymes as well as adaptation to microaerophilic growth conditions play a key role in bacterial survival under biofilm growth conditions. On the other hand, presence of genes involved in motility and PQS signaling were less important during biofilm growth. Several mutants exhibiting transposon insertions in genes detected in our screen were validated for their biofilm growth capabilities and biofilm specific transcriptional responses using independently generated transposon mutants. Our results provide new insights into P. aeruginosa adaptation to biofilm growth conditions. The detection of previously unknown determinants of biofilm survival supports the use of transposon insertion sequencing as a global genomic technology for understanding the establishment of difficult to treat biofilm-associated infections.Citation
Biofilm. 2020 Apr 2;2:100023. doi: 10.1016/j.bioflm.2020.100023.Affiliation
HZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.Publisher
ElsevierJournal
BiofilmPubMed ID
33447809Type
ArticleLanguage
enEISSN
2590-2075ae974a485f413a2113503eed53cd6c53
10.1016/j.bioflm.2020.100023
Scopus Count
The following license files are associated with this item:
- Creative Commons
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