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dc.contributor.authorSchinner, Silvia
dc.contributor.authorEngelhardt, Florian
dc.contributor.authorPreusse, Matthias
dc.contributor.authorThöming, Janne Gesine
dc.contributor.authorTomasch, Jürgen
dc.contributor.authorHäussler, Susanne
dc.date.accessioned2021-02-05T14:21:24Z
dc.date.available2021-02-05T14:21:24Z
dc.date.issued2020-04-02
dc.identifier.citationBiofilm. 2020 Apr 2;2:100023. doi: 10.1016/j.bioflm.2020.100023.en_US
dc.identifier.pmid33447809
dc.identifier.doi10.1016/j.bioflm.2020.100023
dc.identifier.urihttp://hdl.handle.net/10033/622719
dc.description.abstractPseudomonas aeruginosa is an environmental bacterium and an opportunistic human pathogen. It is also a well-established model organism to study bacterial adaptation to stressful conditions, such as those encountered during an infection process in the human host. Advancing knowledge on P. aeruginosa adaptation to biofilm growth conditions is bound to reveal novel strategies and targets for the treatment of chronic biofilm-associated infections. Here, we generated transposon insertion libraries in three P. aeruginosa strain backgrounds and determined the relative frequency of each insertion following biofilm growth using transposon sequencing. We demonstrate that in general the SOS response, several tRNA modifying enzymes as well as adaptation to microaerophilic growth conditions play a key role in bacterial survival under biofilm growth conditions. On the other hand, presence of genes involved in motility and PQS signaling were less important during biofilm growth. Several mutants exhibiting transposon insertions in genes detected in our screen were validated for their biofilm growth capabilities and biofilm specific transcriptional responses using independently generated transposon mutants. Our results provide new insights into P. aeruginosa adaptation to biofilm growth conditions. The detection of previously unknown determinants of biofilm survival supports the use of transposon insertion sequencing as a global genomic technology for understanding the establishment of difficult to treat biofilm-associated infections.en_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relationinfo:eu-repo/grantAgreement/EC/H2020/724290en_US
dc.rightsopenAccessen_US
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectBiofilmen_US
dc.subjectFitness determinantsen_US
dc.subjectPseudomonas aeruginosaen_US
dc.subjectTn-seqen_US
dc.titleGenetic determinants of Pseudomonas aeruginosa fitness during biofilm growth.en_US
dc.typeArticleen_US
dc.identifier.eissn2590-2075
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalBiofilmen_US
dc.source.volume2
dc.source.beginpage100023
dc.source.endpage
refterms.dateFOA2021-02-05T14:21:25Z
dc.source.journaltitleBiofilm
dc.source.countryNetherlands


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