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Collias and Beisel.pdf
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Issue Date
2021-01-22
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Show full item recordAbstract
The ever-expanding set of CRISPR technologies and their programmable RNA-guided nucleases exhibit remarkable flexibility in DNA targeting. However, this flexibility comes with an ever-present constraint: the requirement for a protospacer adjacent motif (PAM) flanking each target. While PAMs play an essential role in self/nonself discrimination by CRISPR-Cas immune systems, this constraint has launched a far-reaching expedition for nucleases with relaxed PAM requirements. Here, we review ongoing efforts toward realizing PAM-free nucleases through natural ortholog mining and protein engineering. We also address potential consequences of fully eliminating PAM recognition and instead propose an alternative nuclease repertoire covering all possible PAM sequences.Citation
Nat Commun. 2021 Jan 22;12(1):555. doi: 10.1038/s41467-020-20633-y.Affiliation
HIRI, Helmholtz-Institut für RNA-basierte Infektionsforschung, Josef-Shneider Strasse 2, 97080 Würzburg, Germany.Publisher
Nature Pulishing GroupJournal
Nature communicationsPubMed ID
33483498Type
ReviewLanguage
enEISSN
2041-1723ae974a485f413a2113503eed53cd6c53
10.1038/s41467-020-20633-y
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- Creative Commons