Second-generation lung-on-a-chip with an array of stretchable alveoli made with a biological membrane.
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Authors
Zamprogno, PaulineWüthrich, Simon
Achenbach, Sven
Thoma, Giuditta
Stucki, Janick D
Hobi, Nina
Schneider-Daum, Nicole
Lehr, Claus-Michael
Huwer, Hanno
Geiser, Thomas
Schmid, Ralph A
Guenat, Olivier T
Issue Date
2021-02-05
Metadata
Show full item recordAbstract
The air-blood barrier with its complex architecture and dynamic environment is difficult to mimic in vitro. Lung-on-a-chips enable mimicking the breathing movements using a thin, stretchable PDMS membrane. However, they fail to reproduce the characteristic alveoli network as well as the biochemical and physical properties of the alveolar basal membrane. Here, we present a lung-on-a-chip, based on a biological, stretchable and biodegradable membrane made of collagen and elastin, that emulates an array of tiny alveoli with in vivo-like dimensions. This membrane outperforms PDMS in many ways: it does not absorb rhodamine-B, is biodegradable, is created by a simple method, and can easily be tuned to modify its thickness, composition and stiffness. The air-blood barrier is reconstituted using primary lung alveolar epithelial cells from patients and primary lung endothelial cells. Typical alveolar epithelial cell markers are expressed, while the barrier properties are preserved for up to 3 weeks.Citation
Commun Biol. 2021 Feb 5;4(1):168. doi: 10.1038/s42003-021-01695-0.Affiliation
HIPS, Helmholtz-Institut für Pharmazeutische Forschung Saarland, Universitätscampus E8.1 66123 Saarbrücken, Germany.Publisher
Nature Pulishing GroupJournal
Communications biologyPubMed ID
33547387Type
ArticleLanguage
enEISSN
2399-3642ae974a485f413a2113503eed53cd6c53
10.1038/s42003-021-01695-0
Scopus Count
The following license files are associated with this item:
- Creative Commons
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