Show simple item record

dc.contributor.authorHasenzahl, Meike
dc.contributor.authorMüsken, Mathias
dc.contributor.authorMertsch, Sonja
dc.contributor.authorSchrader, Stefan
dc.contributor.authorReichl, Stephan
dc.date.accessioned2021-03-02T13:18:12Z
dc.date.available2021-03-02T13:18:12Z
dc.date.issued2021-02-03
dc.identifier.citationJ Biomed Mater Res B Appl Biomater. 2021 Feb 3. doi: 10.1002/jbm.b.34808. Epub ahead of print.en_US
dc.identifier.pmid33538123
dc.identifier.doi10.1002/jbm.b.34808
dc.identifier.urihttp://hdl.handle.net/10033/622763
dc.description.abstractThe in vitro reconstruction of stromal tissue by long-term cultivation of corneal fibroblasts is a smart approach for regenerative therapies of ocular surface diseases. However, systematic investigations evaluating optimized cultivation protocols for the realization of a biomaterial are lacking. This study investigated the influence of supplements to the culture media of human corneal fibroblasts on the formation of a cell sheet consisting of cells and extracellular matrix. Among the supplements studied are vitamin C, fetal bovine serum, L-glutamine, components of collagen such as L-proline, L-4-hydroxyproline and glycine, and TGF-β1, bFGF, IGF-2, PDGF-BB and insulin. After long-term cultivation, the proliferation, collagen and glycosaminoglycan content and light transmission of the cell sheets were examined. Biomechanical properties were investigated by tensile tests and the ultrastructure was characterized by electron microscopy, small-angle X-ray scattering, antibody staining and ELISA. The synthesis of extracellular matrix was significantly increased by cultivation with insulin or TGF-β1, each with vitamin C. The sheets exhibited a high transparency and suitable material properties. The production of a transparent, scaffold-free, potentially autologous, in vitro-generated construct by culturing fibroblasts with extracellular matrix synthesis-stimulating supplements represents a promising approach for a biomaterial that can be used for ocular surface reconstruction in slowly progressing diseases.en_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectcorneal tissue engineeringen_US
dc.subjectgrowth factorsen_US
dc.subjectkeratocytesen_US
dc.subjectocular surface reconstructionen_US
dc.subjectvitamin Cen_US
dc.titleCell sheet technology: Influence of culture conditions on in vitro-cultivated corneal stromal tissue for regenerative therapies of the ocular surface.en_US
dc.typeArticleen_US
dc.identifier.eissn1552-4981
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalJournal of biomedical materials research. Part B, Applied biomaterialsen_US
refterms.dateFOA2021-03-02T13:18:13Z
dc.source.journaltitleJournal of biomedical materials research. Part B, Applied biomaterials
dc.source.countryUnited States


Files in this item

Thumbnail
Name:
Hasenzahl et al.pdf
Size:
3.755Mb
Format:
PDF
Description:
Open Access publication

This item appears in the following Collection(s)

Show simple item record

Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International