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dc.contributor.authorCorreia Santos, Sara
dc.contributor.authorBischler, Thorsten
dc.contributor.authorWestermann, Alexander J
dc.contributor.authorVogel, Jörg
dc.date.accessioned2021-03-04T12:25:27Z
dc.date.available2021-03-04T12:25:27Z
dc.date.issued2021-02-02
dc.identifier.citationCell Rep. 2021 Feb 2;34(5):108722. doi: 10.1016/j.celrep.2021.108722.en_US
dc.identifier.pmid33535041
dc.identifier.doi10.1016/j.celrep.2021.108722
dc.identifier.urihttp://hdl.handle.net/10033/622769
dc.description.abstractA full understanding of the contribution of small RNAs (sRNAs) to bacterial virulence demands knowledge of their target suites under infection-relevant conditions. Here, we take an integrative approach to capturing targets of the Hfq-associated sRNA PinT, a known post-transcriptional timer of the two major virulence programs of Salmonella enterica. Using MS2 affinity purification and RNA sequencing (MAPS), we identify PinT ligands in bacteria under in vitro conditions mimicking specific stages of the infection cycle and in bacteria growing inside macrophages. This reveals PinT-mediated translational inhibition of the secreted effector kinase SteC, which had gone unnoticed in previous target searches. Using genetic, biochemical, and microscopic assays, we provide evidence for PinT-mediated repression of steC mRNA, eventually delaying actin rearrangements in infected host cells. Our findings support the role of PinT as a central post-transcriptional regulator in Salmonella virulence and illustrate the need for complementary methods to reveal the full target suites of sRNAs.en_US
dc.language.isoenen_US
dc.publisherElsevier (Cell Press)en_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectMS2 affinity purification and RNA-seqen_US
dc.subjectPinTen_US
dc.subjecthost-pathogen interactionen_US
dc.subjectinfectionen_US
dc.subjectnoncoding RNAen_US
dc.subjectpost-transcriptional controlen_US
dc.titleMAPS integrates regulation of actin-targeting effector SteC into the virulence control network of Salmonella small RNA PinT.en_US
dc.typeArticleen_US
dc.identifier.eissn2211-1247
dc.contributor.departmentHIRI, Helmholtz-Institut für RNA-basierte Infektionsforschung, Josef-Shneider Strasse 2, 97080 Würzburg, Germany.en_US
dc.identifier.journalCell reportsen_US
dc.source.volume34
dc.source.issue5
dc.source.beginpage108722
dc.source.endpage
refterms.dateFOA2021-03-04T12:25:28Z
dc.source.journaltitleCell reports
dc.source.countryUnited States


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International