The Two-Component System 09 Regulates Pneumococcal Carbohydrate Metabolism and Capsule Expression.
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Authors
Hirschmann, StephanieGómez-Mejia, Alejandro
Mäder, Ulrike
Karsunke, Julia
Driesch, Dominik
Rohde, Manfred
Häussler, Susanne
Burchhardt, Gerhard
Hammerschmidt, Sven
Issue Date
2021-02-24
Metadata
Show full item recordAbstract
Streptococcus pneumoniae two-component regulatory systems (TCSs) are important systems that perceive and respond to various host environmental stimuli. In this study, we have explored the role of TCS09 on gene expression and phenotypic alterations in S. pneumoniae D39. Our comparative transcriptomic analyses identified 67 differently expressed genes in total. Among those, agaR and the aga operon involved in galactose metabolism showed the highest changes. Intriguingly, the encapsulated and nonencapsulated hk09-mutants showed significant growth defects under nutrient-defined conditions, in particular with galactose as a carbon source. Phenotypic analyses revealed alterations in the morphology of the nonencapsulated hk09- and tcs09-mutants, whereas the encapsulated hk09- and tcs09-mutants produced higher amounts of capsule. Interestingly, the encapsulated D39∆hk09 showed only the opaque colony morphology, while the D39∆rr09- and D39∆tcs09-mutants had a higher proportion of transparent variants. The phenotypic variations of D39ΔcpsΔhk09 and D39ΔcpsΔtcs09 are in accordance with their higher numbers of outer membrane vesicles, higher sensitivity against Triton X-100 induced autolysis, and lower resistance against oxidative stress. In conclusion, these results indicate the importance of TCS09 for pneumococcal metabolic fitness and resistance against oxidative stress by regulating the carbohydrate metabolism and thereby, most likely indirectly, the cell wall integrity and amount of capsular polysaccharide.Citation
Microorganisms. 2021 Feb 24;9(3):468. doi: 10.3390/microorganisms9030468.Affiliation
HZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.Publisher
MDPIJournal
MicroorganismsPubMed ID
33668344Type
ArticleLanguage
enISSN
2076-2607ae974a485f413a2113503eed53cd6c53
10.3390/microorganisms9030468
Scopus Count
The following license files are associated with this item:
- Creative Commons
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