Show simple item record

dc.contributor.authorHirschmann, Stephanie
dc.contributor.authorGómez-Mejia, Alejandro
dc.contributor.authorMäder, Ulrike
dc.contributor.authorKarsunke, Julia
dc.contributor.authorDriesch, Dominik
dc.contributor.authorRohde, Manfred
dc.contributor.authorHäussler, Susanne
dc.contributor.authorBurchhardt, Gerhard
dc.contributor.authorHammerschmidt, Sven
dc.date.accessioned2021-04-07T14:38:46Z
dc.date.available2021-04-07T14:38:46Z
dc.date.issued2021-02-24
dc.identifier.citationMicroorganisms. 2021 Feb 24;9(3):468. doi: 10.3390/microorganisms9030468.en_US
dc.identifier.issn2076-2607
dc.identifier.pmid33668344
dc.identifier.doi10.3390/microorganisms9030468
dc.identifier.urihttp://hdl.handle.net/10033/622824
dc.description.abstractStreptococcus pneumoniae two-component regulatory systems (TCSs) are important systems that perceive and respond to various host environmental stimuli. In this study, we have explored the role of TCS09 on gene expression and phenotypic alterations in S. pneumoniae D39. Our comparative transcriptomic analyses identified 67 differently expressed genes in total. Among those, agaR and the aga operon involved in galactose metabolism showed the highest changes. Intriguingly, the encapsulated and nonencapsulated hk09-mutants showed significant growth defects under nutrient-defined conditions, in particular with galactose as a carbon source. Phenotypic analyses revealed alterations in the morphology of the nonencapsulated hk09- and tcs09-mutants, whereas the encapsulated hk09- and tcs09-mutants produced higher amounts of capsule. Interestingly, the encapsulated D39∆hk09 showed only the opaque colony morphology, while the D39∆rr09- and D39∆tcs09-mutants had a higher proportion of transparent variants. The phenotypic variations of D39ΔcpsΔhk09 and D39ΔcpsΔtcs09 are in accordance with their higher numbers of outer membrane vesicles, higher sensitivity against Triton X-100 induced autolysis, and lower resistance against oxidative stress. In conclusion, these results indicate the importance of TCS09 for pneumococcal metabolic fitness and resistance against oxidative stress by regulating the carbohydrate metabolism and thereby, most likely indirectly, the cell wall integrity and amount of capsular polysaccharide.en_US
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectStreptococcus pneumoniaeen_US
dc.subjectcapsuleen_US
dc.subjectcarbohydrate metabolismen_US
dc.subjecttwo-component system 09en_US
dc.titleThe Two-Component System 09 Regulates Pneumococcal Carbohydrate Metabolism and Capsule Expression.en_US
dc.typeArticleen_US
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalMicroorganismsen_US
dc.source.volume9
dc.source.issue3
refterms.dateFOA2021-04-07T14:38:46Z
dc.source.journaltitleMicroorganisms
dc.source.countrySwitzerland


Files in this item

Thumbnail
Name:
Hirschmann et al.pdf
Size:
5.606Mb
Format:
PDF
Description:
Open Access publication

This item appears in the following Collection(s)

Show simple item record

Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International