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dc.contributor.authorStinn, Anne
dc.contributor.authorFurkert, Jens
dc.contributor.authorKaufmann, Stefan H E
dc.contributor.authorMoura-Alves, Pedro
dc.contributor.authorKolbe, Michael
dc.date.accessioned2021-04-22T13:28:25Z
dc.date.available2021-04-22T13:28:25Z
dc.date.issued2021-02-24
dc.identifier.citationBiosensors (Basel). 2021 Feb 24;11(3):60. doi: 10.3390/bios11030060.en_US
dc.identifier.pmid33668313
dc.identifier.doi10.3390/bios11030060
dc.identifier.urihttp://hdl.handle.net/10033/622839
dc.description.abstractThe aryl hydrocarbon receptor (AhR) is a highly conserved cellular sensor of a variety of environmental pollutants and dietary-, cell- and microbiota-derived metabolites with important roles in fundamental biological processes. Deregulation of the AhR pathway is implicated in several diseases, including autoimmune diseases and cancer, rendering AhR a promising target for drug development and host-directed therapy. The pharmacological intervention of AhR processes requires detailed information about the ligand binding properties to allow specific targeting of a particular signaling process without affecting the remaining. Here, we present a novel microscale thermophoresis-based approach to monitoring the binding of purified recombinant human AhR to its natural ligands in a cell-free system. This approach facilitates a precise identification and characterization of unknown AhR ligands and represents a screening strategy for the discovery of potential selective AhR modulators.en_US
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectAhRen_US
dc.subjectMSTen_US
dc.subjecthigh-throughput screeningen_US
dc.subjectligand bindingen_US
dc.subjectrecombinant expressionen_US
dc.titleNovel Method for Quantifying AhR-Ligand Binding Affinities Using Microscale Thermophoresis.en_US
dc.typeArticleen_US
dc.identifier.eissn2079-6374
dc.contributor.departmentCSSB, Centre for Structural Systembiologie, Notkestr.85, 22607 Hamburg. Germany.en_US
dc.identifier.journalBiosensorsen_US
dc.source.volume11
dc.source.issue3
refterms.dateFOA2021-04-22T13:28:26Z
dc.source.journaltitleBiosensors
dc.source.countrySwitzerland


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International