Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
AuthorsWhisnant, Adam W
Jürges, Christopher S
Rutkowski, Andrzej J
Matheson, Nicholas J
Künzig, Florian W H
Lehner, Paul J
Friedel, Caroline C
MetadataShow full item record
AbstractThe predicted 80 open reading frames (ORFs) of herpes simplex virus 1 (HSV-1) have been intensively studied for decades. Here, we unravel the complete viral transcriptome and translatome during lytic infection with base-pair resolution by computational integration of multi-omics data. We identify a total of 201 transcripts and 284 ORFs including all known and 46 novel large ORFs. This includes a so far unknown ORF in the locus deleted in the FDA-approved oncolytic virus Imlygic. Multiple transcript isoforms expressed from individual gene loci explain translation of the vast majority of ORFs as well as N-terminal extensions (NTEs) and truncations. We show that NTEs with non-canonical start codons govern the subcellular protein localization and packaging of key viral regulators and structural proteins. We extend the current nomenclature to include all viral gene products and provide a genome browser that visualizes all the obtained data from whole genome to single-nucleotide resolution.
CitationNat Commun. 2020 Apr 27;11(1):2038. doi: 10.1038/s41467-020-15992-5.
AffiliationHIRI, Helmholtz-Institut für RNA-basierte Infektionsforschung, Josef-Shneider Strasse 2, 97080 Würzburg, Germany.
The following license files are associated with this item:
- Creative Commons
- A protein encoded by the herpes simplex virus (HSV) type 1 2-kilobase latency-associated transcript is phosphorylated, localized to the nucleus, and overcomes the repression of expression from exogenous promoters when inserted into the quiescent HSV genome.
- Authors: Thomas SK, Lilley CE, Latchman DS, Coffin RS
- Issue date: 2002 Apr
- A virus with a mutation in the ICP4-binding site in the L/ST promoter of herpes simplex virus type 1, but not a virus with a mutation in open reading frame P, exhibits cell-type-specific expression of gamma(1)34.5 transcripts and latency-associated transcripts.
- Authors: Lee LY, Schaffer PA
- Issue date: 1998 May
- Transcriptional mapping of the varicella-zoster virus regulatory genes encoding open reading frames 4 and 63.
- Authors: Kinchington PR, Vergnes JP, Defechereux P, Piette J, Turse SE
- Issue date: 1994 Jun
- Role of the Herpes Simplex Virus CVSC Proteins at the Capsid Portal Vertex.
- Authors: Huet A, Huffman JB, Conway JF, Homa FL
- Issue date: 2020 Nov 23
- Transcriptional analysis of the region of the herpes simplex virus type 1 genome containing the UL8, UL9, and UL10 genes and identification of a novel delayed-early gene product, OBPC.
- Authors: Baradaran K, Dabrowski CE, Schaffer PA
- Issue date: 1994 Jul