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dc.contributor.authorRyan, Daniel
dc.contributor.authorJenniches, Laura
dc.contributor.authorReichardt, Sarah
dc.contributor.authorBarquist, Lars
dc.contributor.authorWestermann, Alexander J
dc.date.accessioned2021-04-27T12:31:54Z
dc.date.available2021-04-27T12:31:54Z
dc.date.issued2020-07-16
dc.identifier.citationNat Commun. 2020 Jul 16;11(1):3557. doi: 10.1038/s41467-020-17348-5.en_US
dc.identifier.pmid32678091
dc.identifier.doi10.1038/s41467-020-17348-5
dc.identifier.urihttp://hdl.handle.net/10033/622846
dc.description.abstractBacteria of the genus Bacteroides are common members of the human intestinal microbiota and important degraders of polysaccharides in the gut. Among them, the species Bacteroides thetaiotaomicron has emerged as the model organism for functional microbiota research. Here, we use differential RNA sequencing (dRNA-seq) to generate a single-nucleotide resolution transcriptome map of B. thetaiotaomicron grown under defined laboratory conditions. An online browser, called 'Theta-Base' ( www.helmholtz-hiri.de/en/datasets/bacteroides ), is launched to interrogate the obtained gene expression data and annotations of ~4500 transcription start sites, untranslated regions, operon structures, and 269 noncoding RNA elements. Among the latter is GibS, a conserved, 145 nt-long small RNA that is highly expressed in the presence of N-acetyl-D-glucosamine as sole carbon source. We use computational predictions and experimental data to determine the secondary structure of GibS and identify its target genes. Our results indicate that sensing of N-acetyl-D-glucosamine induces GibS expression, which in turn modifies the transcript levels of metabolic enzymes.en_US
dc.language.isoenen_US
dc.publisherNPGen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleA high-resolution transcriptome map identifies small RNA regulation of metabolism in the gut microbe Bacteroides thetaiotaomicron.en_US
dc.typeArticleen_US
dc.identifier.eissn2041-1723
dc.contributor.departmentHIRI, Helmholtz-Institut für RNA-basierte Infektionsforschung, Josef-Shneider Strasse 2, 97080 Würzburg, Germany.en_US
dc.identifier.journalNature communicationsen_US
dc.source.volume11
dc.source.issue1
dc.source.beginpage3557
dc.source.endpage
refterms.dateFOA2021-04-27T12:31:55Z
dc.source.journaltitleNature communications
dc.source.countryEngland


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International