Structural Basis for Designing Multiepitope Vaccines Against COVID-19 Infection: In Silico Vaccine Design and Validation.
dc.contributor.author | Srivastava, Sukrit | |
dc.contributor.author | Verma, Sonia | |
dc.contributor.author | Kamthania, Mohit | |
dc.contributor.author | Kaur, Rupinder | |
dc.contributor.author | Badyal, Ruchi Kiran | |
dc.contributor.author | Saxena, Ajay Kumar | |
dc.contributor.author | Shin, Ho-Joon | |
dc.contributor.author | Kolbe, Michael | |
dc.contributor.author | Pandey, Kailash C | |
dc.date.accessioned | 2021-04-30T13:18:28Z | |
dc.date.available | 2021-04-30T13:18:28Z | |
dc.date.issued | 2020-06-19 | |
dc.identifier.citation | JMIR Bioinform Biotech. 2020 Jun 19;1(1):e19371. doi: 10.2196/19371. | en_US |
dc.identifier.pmid | 32776022 | |
dc.identifier.doi | 10.2196/19371 | |
dc.identifier.uri | http://hdl.handle.net/10033/622852 | |
dc.description.abstract | Both designed MEVs are composed of CTL and HTL epitopes screened from 11 Open Reading Frame (ORF), structural and nonstructural proteins of the SARS-CoV-2 proteome. Both MEVs also carry potential B-cell linear and discontinuous epitopes as well as interferon gamma-inducing epitopes. To enhance the immune response of our vaccine design, truncated (residues 10-153) Onchocerca volvulus activation-associated secreted protein-1 was used as an adjuvant at the N termini of both MEVs. The tertiary models for both the designed MEVs were generated, refined, and further analyzed for stable molecular interaction with toll-like receptor 3. Codon-biased complementary DNA (cDNA) was generated for both MEVs and analyzed in silico for high level expression in a mammalian (human) host cell line. | en_US |
dc.language.iso | en | en_US |
dc.publisher | : JMIR Publications Inc. | en_US |
dc.rights | Attribution 4.0 International | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | COVID-19 | en_US |
dc.subject | coronavirus | en_US |
dc.subject | epitope | en_US |
dc.subject | human transporter associated with antigen processing (TAP) | en_US |
dc.subject | immunoinformatics | en_US |
dc.subject | molecular docking, molecular dynamics simulation | en_US |
dc.subject | multiepitope vaccine | en_US |
dc.subject | severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) | en_US |
dc.subject | toll-like receptor (TLR) | en_US |
dc.title | Structural Basis for Designing Multiepitope Vaccines Against COVID-19 Infection: In Silico Vaccine Design and Validation. | en_US |
dc.type | Article | en_US |
dc.identifier.eissn | 2563-3570 | |
dc.contributor.department | CSSB, Centre for Structural Systembiologie, Notkestr.85, 22607 Hamburg. Germany. | en_US |
dc.identifier.journal | JMIR bioinformatics and biotechnology | en_US |
dc.source.volume | 1 | |
dc.source.issue | 1 | |
dc.source.beginpage | e19371 | |
dc.source.endpage | ||
refterms.dateFOA | 2021-04-30T13:18:29Z | |
dc.source.journaltitle | JMIR bioinformatics and biotechnology | |
dc.source.country | Canada |