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dc.contributor.authorBernardes, Joana P
dc.contributor.authorMishra, Neha
dc.contributor.authorTran, Florian
dc.contributor.authorBahmer, Thomas
dc.contributor.authorBest, Lena
dc.contributor.authorBlase, Johanna I
dc.contributor.authorBordoni, Dora
dc.contributor.authorFranzenburg, Jeanette
dc.contributor.authorGeisen, Ulf
dc.contributor.authorJosephs-Spaulding, Jonathan
dc.contributor.authorKöhler, Philipp
dc.contributor.authorKünstner, Axel
dc.contributor.authorRosati, Elisa
dc.contributor.authorAschenbrenner, Anna C
dc.contributor.authorBacher, Petra
dc.contributor.authorBaran, Nathan
dc.contributor.authorBoysen, Teide
dc.contributor.authorBrandt, Burkhard
dc.contributor.authorBruse, Niklas
dc.contributor.authorDörr, Jonathan
dc.contributor.authorDräger, Andreas
dc.contributor.authorElke, Gunnar
dc.contributor.authorEllinghaus, David
dc.contributor.authorFischer, Julia
dc.contributor.authorForster, Michael
dc.contributor.authorFranke, Andre
dc.contributor.authorFranzenburg, Sören
dc.contributor.authorFrey, Norbert
dc.contributor.authorFriedrichs, Anette
dc.contributor.authorFuß, Janina
dc.contributor.authorGlück, Andreas
dc.contributor.authorHamm, Jacob
dc.contributor.authorHinrichsen, Finn
dc.contributor.authorHoeppner, Marc P
dc.contributor.authorImm, Simon
dc.contributor.authorJunker, Ralf
dc.contributor.authorKaiser, Sina
dc.contributor.authorKan, Ying H
dc.contributor.authorKnoll, Rainer
dc.contributor.authorLange, Christoph
dc.contributor.authorLaue, Georg
dc.contributor.authorLier, Clemens
dc.contributor.authorLindner, Matthias
dc.contributor.authorMarinos, Georgios
dc.contributor.authorMarkewitz, Robert
dc.contributor.authorNattermann, Jacob
dc.contributor.authorNoth, Rainer
dc.contributor.authorPickkers, Peter
dc.contributor.authorRabe, Klaus F
dc.contributor.authorRenz, Alina
dc.contributor.authorRöcken, Christoph
dc.contributor.authorRupp, Jan
dc.contributor.authorSchaffarzyk, Annika
dc.contributor.authorScheffold, Alexander
dc.contributor.authorSchulte-Schrepping, Jonas
dc.contributor.authorSchunk, Domagoj
dc.contributor.authorSkowasch, Dirk
dc.contributor.authorUlas, Thomas
dc.contributor.authorWandinger, Klaus-Peter
dc.contributor.authorWittig, Michael
dc.contributor.authorZimmermann, Johannes
dc.contributor.authorBusch, Hauke
dc.contributor.authorHoyer, Bimba F
dc.contributor.authorKaleta, Christoph
dc.contributor.authorHeyckendorf, Jan
dc.contributor.authorKox, Matthijs
dc.contributor.authorRybniker, Jan
dc.contributor.authorSchreiber, Stefan
dc.contributor.authorSchultze, Joachim L
dc.contributor.authorRosenstiel, Philip
dc.contributor.authorDeutsche COVID-19 Omics Initiative (DeCOI)
dc.date.accessioned2021-05-05T11:04:51Z
dc.date.available2021-05-05T11:04:51Z
dc.date.issued2020-11-26
dc.identifier.citationmmunity. 2020 Dec 15;53(6):1296-1314.e9. doi: 10.1016/j.immuni.2020.11.017. Epub 2020 Nov 26.en_US
dc.identifier.pmid33296687
dc.identifier.doi10.1016/j.immuni.2020.11.017
dc.identifier.urihttp://hdl.handle.net/10033/622854
dc.description.abstractTemporal resolution of cellular features associated with a severe COVID-19 disease trajectory is needed for understanding skewed immune responses and defining predictors of outcome. Here, we performed a longitudinal multi-omics study using a two-center cohort of 14 patients. We analyzed the bulk transcriptome, bulk DNA methylome, and single-cell transcriptome (>358,000 cells, including BCR profiles) of peripheral blood samples harvested from up to 5 time points. Validation was performed in two independent cohorts of COVID-19 patients. Severe COVID-19 was characterized by an increase of proliferating, metabolically hyperactive plasmablasts. Coinciding with critical illness, we also identified an expansion of interferon-activated circulating megakaryocytes and increased erythropoiesis with features of hypoxic signaling. Megakaryocyte- and erythroid-cell-derived co-expression modules were predictive of fatal disease outcome. The study demonstrates broad cellular effects of SARS-CoV-2 infection beyond adaptive immune cells and provides an entry point toward developing biomarkers and targeted treatments of patients with COVID-19.en_US
dc.language.isoenen_US
dc.publisherElsevier (Cell Press)en_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectCOVID-19en_US
dc.subjectRNA-seqen_US
dc.subjectacute respiratory distressen_US
dc.subjectblooden_US
dc.subjectdisease trajectoryen_US
dc.subjectimmune responseen_US
dc.subjectinfectious diseaseen_US
dc.subjectmethylationen_US
dc.subjectscRNA-seqen_US
dc.subjectvirusen_US
dc.titleLongitudinal Multi-omics Analyses Identify Responses of Megakaryocytes, Erythroid Cells, and Plasmablasts as Hallmarks of Severe COVID-19.en_US
dc.typeArticleen_US
dc.identifier.eissn1097-4180
dc.contributor.departmentHIRI, Helmholtz-Institut für RNA-basierte Infektionsforschung, Josef-Shneider Strasse 2, 97080 Würzburg, Germany.; BRICS, Braunschweiger Zentrum für Systembiologie, Rebenring 56,38106 Braunschweig, Germany.en_US
dc.identifier.journalImmunityen_US
dc.source.volume53
dc.source.issue6
dc.source.beginpage1296
dc.source.endpage1314.e9
refterms.dateFOA2021-05-05T11:04:51Z
dc.source.journaltitleImmunity
dc.source.countryUnited States


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International