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dc.contributor.authorDomínguez-Pérez, Dany
dc.contributor.authorAlmeida, Daniela
dc.contributor.authorWissing, Josef
dc.contributor.authorMachado, André M
dc.contributor.authorJänsch, Lothar
dc.contributor.authorAntunes, Agostinho
dc.contributor.authorCastro, Luís Filipe
dc.contributor.authorVasconcelos, Vitor
dc.contributor.authorCampos, Alexandre
dc.contributor.authorCunha, Isabel
dc.date.accessioned2021-05-05T14:23:02Z
dc.date.available2021-05-05T14:23:02Z
dc.date.issued2021-03-25
dc.identifier.citationInt J Mol Sci. 2021 Mar 25;22(7):3370. doi: 10.3390/ijms22073370.en_US
dc.identifier.pmid33806079
dc.identifier.doi10.3390/ijms22073370
dc.identifier.urihttp://hdl.handle.net/10033/622857
dc.description.abstractWe focus on the stalked goose barnacle L. anatifera adhesive system, an opportunistic less selective species for the substrate, found attached to a variety of floating objects at seas. Adhesion is an adaptative character in barnacles, ensuring adequate positioning in the habitat for feeding and reproduction. The protein composition of the cement multicomplex and adhesive gland was quantitatively studied using shotgun proteomic analysis. Overall, 11,795 peptide sequences were identified in the gland and 2206 in the cement, clustered in 1689 and 217 proteinGroups, respectively. Cement specific adhesive proteins (CPs), proteases, protease inhibitors, cuticular and structural proteins, chemical cues, and many unannotated proteins were found, among others. In the cement, CPs were the most abundant (80.5%), being the bulk proteins CP100k and -52k the most expressed of all, and CP43k-like the most expressed interfacial protein. Unannotated proteins comprised 4.7% of the cement proteome, ranking several of them among the most highly expressed. Eight of these proteins showed similar physicochemical properties and amino acid composition to known CPs and classified through Principal Components Analysis (PCA) as new CPs. The importance of PCA on the identification of unannotated non-conserved adhesive proteins, whose selective pressure is on their relative amino acid abundance, was demonstrated.en_US
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectcement proteinen_US
dc.subjectiBAQen_US
dc.subjectmass spectrometryen_US
dc.subjectprotein expressionen_US
dc.subjectshotgun proteomicsen_US
dc.subjectunderwater adhesionen_US
dc.titleProteogenomic Characterization of the Cement and Adhesive Gland of the Pelagic Gooseneck Barnacle .en_US
dc.typeArticleen_US
dc.identifier.eissn1422-0067
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalInternational journal of molecular sciencesen_US
dc.source.volume22
dc.source.issue7
refterms.dateFOA2021-05-05T14:23:03Z
dc.source.journaltitleInternational journal of molecular sciences
dc.source.countrySwitzerland


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International