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dc.contributor.authorFráguas-Eggenschwiler, Mariane
dc.contributor.authorEggenschwiler, Reto
dc.contributor.authorSöllner, Jenny-Helena
dc.contributor.authorCortnumme, Leon
dc.contributor.authorVondran, Florian W R
dc.contributor.authorCantz, Tobias
dc.contributor.authorOtt, Michael
dc.contributor.authorNiemann, Heiner
dc.date.accessioned2021-05-17T09:57:12Z
dc.date.available2021-05-17T09:57:12Z
dc.date.issued2021-04-29
dc.identifier.citationSci Rep. 2021 Apr 29;11(1):9334. doi: 10.1038/s41598-021-88727-1.en_US
dc.identifier.pmid33927320
dc.identifier.doi10.1038/s41598-021-88727-1
dc.identifier.urihttp://hdl.handle.net/10033/622872
dc.description.abstractThe pig is an important model organism for biomedical research, mainly due to its extensive genetic, physiological and anatomical similarities with humans. Until date, direct conversion of somatic cells into hepatocyte-like cells (iHeps) has only been achieved in rodents and human cells. Here, we employed lentiviral vectors to screen a panel of 12 hepatic transcription factors (TF) for their potential to convert porcine fibroblasts into hepatocyte-like cells. We demonstrate for the first time, hepatic conversion of porcine somatic cells by over-expression of CEBPα, FOXA1 and HNF4α2 (3TF-piHeps). Reprogrammed 3TF-piHeps display a hepatocyte-like morphology and show functional characteristics of hepatic cells, including albumin secretion, Dil-AcLDL uptake, storage of lipids and glycogen and activity of cytochrome P450 enzymes CYP1A2 and CYP2C33 (CYP2C9 in humans). Moreover, we show that markers of mature hepatocytes are highly expressed in 3TF-piHeps, while fibroblastic markers are reduced. We envision piHeps as useful cell sources for future studies on drug metabolism and toxicity as well as in vitro models for investigation of pig-to-human infectious diseases.en_US
dc.language.isoenen_US
dc.publisherNature Researchen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleDirect conversion of porcine primary fibroblasts into hepatocyte-like cells.en_US
dc.typeArticleen_US
dc.identifier.eissn2045-2322
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalScientific reportsen_US
dc.source.volume11
dc.source.issue1
dc.source.beginpage9334
dc.source.endpage
refterms.dateFOA2021-05-17T09:57:13Z
dc.source.journaltitleScientific reports
dc.source.countryEngland


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International