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dc.contributor.authorRahman, Shah Kamranur
dc.contributor.authorAnsari, Mairaj Ahmed
dc.contributor.authorGaur, Pratibha
dc.contributor.authorAhmad, Imtiyaz
dc.contributor.authorChakravarty, Chandrani
dc.contributor.authorVerma, Dileep Kumar
dc.contributor.authorSharma, Anshika
dc.contributor.authorChhibber, Sanjay
dc.contributor.authorNehal, Naila
dc.contributor.authorWirth, Dagmar
dc.contributor.authorLal, Sunil K
dc.date.accessioned2021-05-17T12:08:28Z
dc.date.available2021-05-17T12:08:28Z
dc.date.issued2021-04-21
dc.identifier.citationViruses. 2021 Apr 21;13(5):726. doi: 10.3390/v13050726.en_US
dc.identifier.pmid33919410
dc.identifier.doi10.3390/v13050726
dc.identifier.urihttp://hdl.handle.net/10033/622873
dc.description.abstractTo establish a productive infection in host cells, viruses often use one or multiple host membrane glycoproteins as their receptors. For Influenza A virus (IAV) such a glycoprotein receptor has not been described, to date. Here we show that IAV is using the host membrane glycoprotein CD66c as a receptor for entry into human epithelial lung cells. Neuraminidase (NA), a viral spike protein, binds to CD66c on the cell surface during IAV entry into the host cells. Lung cells overexpressing CD66c showed an increase in virus binding and subsequent entry into the cell. Upon comparison, CD66c demonstrated higher binding capacity than other membrane glycoproteins (EGFR and DC-SIGN) reported earlier to facilitate IAV entry into host cells. siRNA mediated knockdown of CD66c from lung cells inhibited virus binding on cell surface and entry into cells. Blocking CD66c by antibody on the cell surface resulted in decreased virus entry. We found that CD66c is a specific glycoprotein receptor for influenza A virus that did not affect entry of non-IAV RNA virus (Hepatitis C virus). Finally, IAV pre-incubated with recombinant CD66c protein when administered intranasally in mice showed decreased cytopathic effects in mice lungs. This publication is the first to report CD66c (Carcinoembryonic cell adhesion molecule 6 or CEACAM6) as a glycoprotein receptor for Influenza A virus.en_US
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectCD66cen_US
dc.subjectCEACAM6en_US
dc.subjectIgG super familyen_US
dc.subjectcarcinoembryonic antigen (CEA)en_US
dc.subjectcarcinoembryonic cell adhesion molecule (CEACAM)en_US
dc.subjectfluen_US
dc.subjecthemagglutininen_US
dc.subjectinfluenza A Virusen_US
dc.subjectlipid raften_US
dc.subjectneuraminidaseen_US
dc.subjectreceptoren_US
dc.subjectsialic aciden_US
dc.subjectvirusen_US
dc.titleThe Immunomodulatory CEA Cell Adhesion Molecule 6 (CEACAM6/CD66c) Is a Protein Receptor for the Influenza a Virus.en_US
dc.typeArticleen_US
dc.identifier.eissn1999-4915
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalVirusesen_US
dc.source.volume13
dc.source.issue5
refterms.dateFOA2021-05-17T12:08:28Z
dc.source.journaltitleViruses
dc.source.countrySwitzerland


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International