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dc.contributor.authorEl Mouali, Youssef
dc.contributor.authorGerovac, Milan
dc.contributor.authorMineikaitė, Raminta
dc.contributor.authorVogel, Jörg
dc.date.accessioned2021-05-17T14:13:57Z
dc.date.available2021-05-17T14:13:57Z
dc.date.issued2021-05-03
dc.identifier.citationNucleic Acids Res. 2021 May 3:gkab281. doi: 10.1093/nar/gkab281. Epub ahead of print.en_US
dc.identifier.pmid33939833
dc.identifier.doi10.1093/nar/gkab281
dc.identifier.urihttp://hdl.handle.net/10033/622875
dc.description.abstractFinO-domain proteins represent an emerging family of RNA-binding proteins (RBPs) with diverse roles in bacterial post-transcriptional control and physiology. They exhibit an intriguing targeting spectrum, ranging from an assumed single RNA pair (FinP/traJ) for the plasmid-encoded FinO protein, to transcriptome-wide activity as documented for chromosomally encoded ProQ proteins. Thus, the shared FinO domain might bear an unusual plasticity enabling it to act either selectively or promiscuously on the same cellular RNA pool. One caveat to this model is that the full suite of in vivo targets of the assumedly highly selective FinO protein is unknown. Here, we have extensively profiled cellular transcripts associated with the virulence plasmid-encoded FinO in Salmonella enterica. While our analysis confirms the FinP sRNA of plasmid pSLT as the primary FinO target, we identify a second major ligand: the RepX sRNA of the unrelated antibiotic resistance plasmid pRSF1010. FinP and RepX are strikingly similar in length and structure, but not in primary sequence, and so may provide clues to understanding the high selectivity of FinO-RNA interactions. Moreover, we observe that the FinO RBP encoded on the Salmonella virulence plasmid controls the replication of a cohabitating antibiotic resistance plasmid, suggesting cross-regulation of plasmids on the RNA level.en_US
dc.language.isoenen_US
dc.publisherOxford Academicen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleIn vivo targets of Salmonella FinO include a FinP-like small RNA controlling copy number of a cohabitating plasmid.en_US
dc.typeArticleen_US
dc.identifier.eissn1362-4962
dc.contributor.departmentHIRI, Helmholtz-Institut für RNA-basierte Infektionsforschung, Josef-Shneider Strasse 2, 97080 Würzburg, Germany.en_US
dc.identifier.journalNucleic acids researchen_US
refterms.dateFOA2021-05-17T14:13:57Z
dc.source.journaltitleNucleic acids research
dc.source.countryEngland


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International