Show simple item record

dc.contributor.authorZayed, Ashraf R
dc.contributor.authorPecellin, Marina
dc.contributor.authorJaber, Lina
dc.contributor.authorButmeh, Suha
dc.contributor.authorBahader, Shereen A
dc.contributor.authorSteinert, Michael
dc.contributor.authorHöfle, Manfred G
dc.contributor.authorBrettar, Ingrid
dc.contributor.authorBitar, Dina M
dc.date.accessioned2021-05-18T14:15:09Z
dc.date.available2021-05-18T14:15:09Z
dc.date.issued2021-04-01
dc.identifier.citationPathogens. 2021 Apr 1;10(4):417. doi: 10.3390/pathogens10040417.en_US
dc.identifier.issn2076-0817
dc.identifier.pmid33915921
dc.identifier.doi10.3390/pathogens10040417
dc.identifier.urihttp://hdl.handle.net/10033/622878
dc.description.abstractLegionella pneumophila is the causative agent of Legionnaires' disease. Due to the hot climate and intermittent water supply, the West Bank, Palestine, can be considered a high-risk area for this often fatal atypical pneumonia. L. pneumophila occurs in biofilms of natural and man-made freshwater environments, where it infects and replicates intracellularly within protozoa. To correlate the genetic diversity of the bacteria in the environment with their virulence properties for protozoan and mammalian host cells, 60 genotyped isolates from hospital water systems in the West Bank were analyzed. The L. pneumophila isolates were previously genotyped by high resolution Multi Locus Variable Number of Tandem Repeat Analysis (MLVA-8(12)) and sorted according to their relationship in clonal complexes (VACC). Strains of relevant genotypes and VACCs were compared according to their capacity to infect Acanthamoeba castellanii and THP-1 macrophages, and to mediate pore-forming cytotoxicity in sheep red blood cells (sRBCs). Based on a previous detailed analysis of the biogeographic distribution and abundance of the MLVA-8(12)-genotypes, the focus of the study was on the most abundant L. pneumophila- genotypes Gt4(17), Gt6 (18) and Gt10(93) and the four relevant clonal complexes [VACC1, VACC2, VACC5 and VACC11]. The highly abundant genotypes Gt4(17) and Gt6(18) are affiliated with VACC1 and sequence type (ST)1 (comprising L. pneumophila str. Paris), and displayed seroroup (Sg)1. Isolates of these two genotypes exhibited significantly higher virulence potentials compared to other genotypes and clonal complexes in the West Bank. Endemic for the West Bank was the clonal complex VACC11 (affiliated with ST461) represented by three relevant genotypes that all displayed Sg6. These genotypes unique for the West Bank showed a lower infectivity and cytotoxicity compared to all other clonal complexes and their affiliated genotypes. Interestingly, the L. pneumophila serotypes ST1 and ST461 were previously identified by in situ-sequence based typing (SBT) as main causative agents of Legionnaires' disease (LD) in the West Bank at a comparable level. Overall, this study demonstrates the site-specific regional diversity of L. pneumophila genotypes in the West Bank and suggests that a combination of MLVA, cellular infection assays and hierarchical agglomerative cluster analysis allows an improved genotype-based risk assessment.en_US
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectGt10(93)en_US
dc.subjectLegionella pneumophilaen_US
dc.subjectMLVAen_US
dc.subjectVACC11en_US
dc.subjectclonal complexen_US
dc.subjectgenotypeen_US
dc.subjectvirulenceen_US
dc.titleCytotoxicity, Intracellular Replication, and Contact-Dependent Pore Formation of Genotyped Environmental Isolates from Hospital Water Systems in the West Bank, Palestine.en_US
dc.typeArticleen_US
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalPathogens (Basel, Switzerland)en_US
dc.source.volume10
dc.source.issue4
refterms.dateFOA2021-05-18T14:15:09Z
dc.source.journaltitlePathogens (Basel, Switzerland)
dc.source.countrySwitzerland


Files in this item

Thumbnail
Name:
Zayed et al.pdf
Size:
1.653Mb
Format:
PDF
Description:
Open Access publication

This item appears in the following Collection(s)

Show simple item record

Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International