The microbiota is dispensable for the early stages of peripheral regulatory T cell induction within mesenteric lymph nodes.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
MetadataShow full item record
AbstractIntestinal Foxp3+ regulatory T cell (Treg) subsets are crucial players in tolerance to microbiota-derived and food-borne antigens, and compelling evidence suggests that the intestinal microbiota modulates their generation, functional specialization, and maintenance. Selected bacterial species and microbiota-derived metabolites, such as short-chain fatty acids (SCFAs), have been reported to promote Treg homeostasis in the intestinal lamina propria. Furthermore, gut-draining mesenteric lymph nodes (mLNs) are particularly efficient sites for the generation of peripherally induced Tregs (pTregs). Despite this knowledge, the direct role of the microbiota and their metabolites in the early stages of pTreg induction within mLNs is not fully elucidated. Here, using an adoptive transfer-based pTreg induction system, we demonstrate that neither transfer of a dysbiotic microbiota nor dietary SCFA supplementation modulated the pTreg induction capacity of mLNs. Even mice housed under germ-free (GF) conditions displayed equivalent pTreg induction within mLNs. Further molecular characterization of these de novo induced pTregs from mLNs by dissection of their transcriptomes and accessible chromatin regions revealed that the microbiota indeed has a limited impact and does not contribute to the initialization of the Treg-specific epigenetic landscape. Overall, our data suggest that the microbiota is dispensable for the early stages of pTreg induction within mLNs.
CitationCell Mol Immunol. 2021 May;18(5):1211-1221. doi: 10.1038/s41423-021-00647-2. Epub 2021 Mar 24.
AffiliationHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.
JournalCellular & molecular immunology
The following license files are associated with this item:
- Creative Commons
- Mesenteric lymph node stromal cell-derived extracellular vesicles contribute to peripheral de novo induction of Foxp3(+) regulatory T cells.
- Authors: Pasztoi M, Pezoldt J, Beckstette M, Lipps C, Wirth D, Rohde M, Paloczi K, Buzas EI, Huehn J
- Issue date: 2017 Dec
- Rapid and Efficient Generation of Regulatory T Cells to Commensal Antigens in the Periphery.
- Authors: Nutsch K, Chai JN, Ai TL, Russler-Germain E, Feehley T, Nagler CR, Hsieh CS
- Issue date: 2016 Sep 27
- Tolerogenic dendritic cells induced the enrichment of CD4(+)Foxp3(+) regulatory T cells via TGF-β in mesenteric lymph nodes of murine LPS-induced tolerance model.
- Authors: Jia L, Lu J, Zhou Y, Tao Y, Xu H, Zheng W, Zhao J, Liang G, Xu L
- Issue date: 2018 Dec
- Neonatally imprinted stromal cell subsets induce tolerogenic dendritic cells in mesenteric lymph nodes.
- Authors: Pezoldt J, Pasztoi M, Zou M, Wiechers C, Beckstette M, Thierry GR, Vafadarnejad E, Floess S, Arampatzi P, Buettner M, Schweer J, Fleissner D, Vital M, Pieper DH, Basic M, Dersch P, Strowig T, Hornef M, Bleich A, Bode U, Pabst O, Bajénoff M, Saliba AE, Huehn J
- Issue date: 2018 Sep 25
- Contribution of Mesenteric Lymph Nodes and GALT to the Intestinal Foxp3+ Regulatory T-Cell Compartment.
- Authors: Geem D, Ngo V, Harusato A, Chassaing B, Gewirtz AT, Newberry RD, Denning TL
- Issue date: 2016 May