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dc.contributor.authorKnoth, Katharina
dc.contributor.authorZäh, Ralf-Kilian
dc.contributor.authorVeldung, Barbara
dc.contributor.authorBurgio, Dominic
dc.contributor.authorWiegand, Birgit
dc.contributor.authorSmola, Hans
dc.contributor.authorBock, Udo
dc.contributor.authorLehr, Claus-Michael
dc.contributor.authorHittinger, Marius
dc.contributor.authorGroß, Henrik
dc.date.accessioned2021-06-07T12:16:04Z
dc.date.available2021-06-07T12:16:04Z
dc.date.issued2021-01-06
dc.identifier.citationSkin Res Technol. 2021 Jan 6. doi: 10.1111/srt.12998. Epub ahead of print.en_US
dc.identifier.pmid33404151
dc.identifier.doi10.1111/srt.12998
dc.identifier.urihttp://hdl.handle.net/10033/622893
dc.description.abstractBackground: In vitro skin permeation experiments are highly relevant for pharmaceutical, cosmetic, agricultural developments, and regulatory evaluation. A key requirement is the skin barrier integrity, that is accompanied by an intact stratum corneum (SC) which implements high skin quality. A variety of integrity tests are currently available, for example, measurement of transepidermal water loss, monitoring the permeation of tritiated water and the measurement of transdermal electrical resistance (TER). Materials and methods: We aimed for a non-destructive examination of barrier integrity as quality control system, based on TER. Therefore, the in-house developed instrument SkinTER measures electrical resistance on excised human skin samples in a non-invasive and easy-to-use pattern. In this proof of concept study, we compared three human in vitro skin models with focus on their TER and permeation properties. The skin integrity was impaired to mimic conditions of skin during age, lifestyle (eg, shaving) or diseases (eg, obesity, psoriasis, and atopic dermatitis). The OECD permeation marker caffeine was correlated to the corresponding TER value. Results: A correlation between both was obtained by having a Pearson coefficient of -0.830. Hereby, a minimum TER value for intact skin samples of ~1.77 kΩ*cm2 was suggested. Intact samples are significantly different (α = ≤0.05) to their impaired counterparts in flux and TER values. Conclusion: The new SkinTER instrument gives a quick and non-invasive feedback on skin quality before a permeation experiment.en_US
dc.language.isoenen_US
dc.publisherWiley & Sonsen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectin vitroen_US
dc.subjectquality controlen_US
dc.subjectskinen_US
dc.subjecttransdermal electrical resistanceen_US
dc.titleDevelopment and evaluation of a quality control system based on transdermal electrical resistance for skin barrier function in vitro.en_US
dc.typeArticleen_US
dc.identifier.eissn1600-0846
dc.contributor.departmentHIPS, Helmholtz-Institut für Pharmazeutische Forschung Saarland, Universitätscampus E8.1 66123 Saarbrücken, Germany.en_US
dc.identifier.journalSkin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)en_US
dc.source.journaltitleSkin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)
dc.source.countryEngland


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