Show simple item record

dc.contributor.authorFloess, Stefan
dc.contributor.authorHuehn, Jochen
dc.date.accessioned2021-06-24T15:34:11Z
dc.date.available2021-06-24T15:34:11Z
dc.date.issued2021-04-30
dc.identifier.citationMethods Mol Biol. 2021;2285:265-276. doi: 10.1007/978-1-0716-1311-5_21.en_US
dc.identifier.pmid33928559
dc.identifier.doi10.1007/978-1-0716-1311-5_21
dc.identifier.urihttp://hdl.handle.net/10033/622910
dc.description.abstractThe comparison of methylomes from immune cells enables the identification of differentially methylated regions and thereby region-associated gene loci. Those regions can be used to discriminate one immune cell population from the other, as well as help to identify key molecules and major pathways determining the unique phenotypes of immune cell lineages. The combination of bisulfite treatment of genomic DNA and next-generation sequencing provides the basis for studying epigenetic changes in different immune cell populations. Further development of whole-genome bisulfite sequencing resulted in a protocol for sequencing libraries that accept both single- or double-stranded DNA from fixed or nonfixed cells, respectively. Therefore, researchers can include immune cell populations in their methylation studies whose isolation depends on the staining of intracellular molecules.en_US
dc.language.isoenen_US
dc.publisherSpringer Natureen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectEpigenetic regulationen_US
dc.subjectMethylomeen_US
dc.subjectSequencing libraryen_US
dc.titleGeneration of Sequencing Libraries for Building Immune Cell Methylomes.en_US
dc.typeArticleen_US
dc.typeBook chapteren_US
dc.identifier.eissn1940-6029
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalMethods in molecular biology (Clifton, N.J.)en_US
dc.source.volume2285
dc.source.beginpage265
dc.source.endpage276
dc.source.journaltitleMethods in molecular biology (Clifton, N.J.)
dc.source.countryUnited States


Files in this item

Thumbnail
Name:
Floess and Hühn.pdf
Size:
450.1Kb
Format:
PDF
Description:
accepted manuscript

This item appears in the following Collection(s)

Show simple item record

Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International