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dc.contributor.authorHayward, Regan J
dc.contributor.authorHumphrys, Michael S
dc.contributor.authorHuston, Wilhelmina M
dc.contributor.authorMyers, Garry S A
dc.date.accessioned2021-07-12T09:25:51Z
dc.date.available2021-07-12T09:25:51Z
dc.date.issued2021-05-17
dc.identifier.citationSci Rep. 2021 May 17;11(1):10399. doi: 10.1038/s41598-021-89921-x.en_US
dc.identifier.pmid34001998
dc.identifier.doi10.1038/s41598-021-89921-x
dc.identifier.urihttp://hdl.handle.net/10033/622933
dc.description.abstractDual RNA-seq experiments examining viral and bacterial pathogens are increasing, but vary considerably in their experimental designs, such as infection rates and RNA depletion methods. Here, we have applied dual RNA-seq to Chlamydia trachomatis infected epithelial cells to examine transcriptomic responses from both organisms. We compared two time points post infection (1 and 24 h), three multiplicity of infection (MOI) ratios (0.1, 1 and 10) and two RNA depletion methods (rRNA and polyA). Capture of bacterial-specific RNA were greatest when combining rRNA and polyA depletion, and when using a higher MOI. However, under these conditions, host RNA capture was negatively impacted. Although it is tempting to use high infection rates, the implications on host cell survival, the potential reduced length of infection cycles and real world applicability should be considered. This data highlights the delicate nature of balancing host-pathogen RNA capture and will assist future transcriptomic-based studies to achieve more specific and relevant infection-related biological insights.en_US
dc.language.isoenen_US
dc.publisherNature Researchen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleDual RNA-seq analysis of in vitro infection multiplicity and RNA depletion methods in Chlamydia-infected epithelial cells.en_US
dc.typeArticleen_US
dc.identifier.eissn2045-2322
dc.contributor.departmentHIRI, Helmholtz-Institut für RNA-basierte Infektionsforschung, Josef-Shneider Strasse 2, 97080 Würzburg, Germany.en_US
dc.identifier.journalScientific reportsen_US
dc.source.volume11
dc.source.issue1
dc.source.beginpage10399
dc.source.endpage
refterms.dateFOA2021-07-12T09:25:52Z
dc.source.journaltitleScientific reports
dc.source.countryEngland


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International