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dc.contributor.authorAltincekic, Nadide
dc.contributor.authorKorn, Sophie Marianne
dc.contributor.authorQureshi, Nusrat Shahin
dc.contributor.authorDujardin, Marie
dc.contributor.authorNinot-Pedrosa, Martí
dc.contributor.authorAbele, Rupert
dc.contributor.authorAbi Saad, Marie Jose
dc.contributor.authorAlfano, Caterina
dc.contributor.authorAlmeida, Fabio C L
dc.contributor.authorAlshamleh, Islam
dc.contributor.authorde Amorim, Gisele Cardoso
dc.contributor.authorAnderson, Thomas K
dc.contributor.authorAnobom, Cristiane D
dc.contributor.authorAnorma, Chelsea
dc.contributor.authorBains, Jasleen Kaur
dc.contributor.authorBax, Adriaan
dc.contributor.authorBlackledge, Martin
dc.contributor.authorBlechar, Julius
dc.contributor.authorBöckmann, Anja
dc.contributor.authorBrigandat, Louis
dc.contributor.authorBula, Anna
dc.contributor.authorBütikofer, Matthias
dc.contributor.authorCamacho-Zarco, Aldo R
dc.contributor.authorCarlomagno, Teresa
dc.contributor.authorCaruso, Icaro Putinhon
dc.contributor.authorCeylan, Betül
dc.contributor.authorChaikuad, Apirat
dc.contributor.authorChu, Feixia
dc.contributor.authorCole, Laura
dc.contributor.authorCrosby, Marquise G
dc.contributor.authorde Jesus, Vanessa
dc.contributor.authorDhamotharan, Karthikeyan
dc.contributor.authorFelli, Isabella C
dc.contributor.authorFerner, Jan
dc.contributor.authorFleischmann, Yanick
dc.contributor.authorFogeron, Marie-Laure
dc.contributor.authorFourkiotis, Nikolaos K
dc.contributor.authorFuks, Christin
dc.contributor.authorFürtig, Boris
dc.contributor.authorGallo, Angelo
dc.contributor.authorGande, Santosh L
dc.contributor.authorGerez, Juan Atilio
dc.contributor.authorGhosh, Dhiman
dc.contributor.authorGomes-Neto, Francisco
dc.contributor.authorGorbatyuk, Oksana
dc.contributor.authorGuseva, Serafima
dc.contributor.authorHacker, Carolin
dc.contributor.authorHäfner, Sabine
dc.contributor.authorHao, Bing
dc.contributor.authorHargittay, Bruno
dc.contributor.authorHenzler-Wildman, K
dc.contributor.authorHoch, Jeffrey C
dc.contributor.authorHohmann, Katharina F
dc.contributor.authorHutchison, Marie T
dc.contributor.authorJaudzems, Kristaps
dc.contributor.authorJović, Katarina
dc.contributor.authorKaderli, Janina
dc.contributor.authorKalniņš, Gints
dc.contributor.authorKaņepe, Iveta
dc.contributor.authorKirchdoerfer, Robert N
dc.contributor.authorKirkpatrick, John
dc.contributor.authorKnapp, Stefan
dc.contributor.authorKrishnathas, Robin
dc.contributor.authorKutz, Felicitas
dc.contributor.authorZur Lage, Susanne
dc.contributor.authorLambertz, Roderick
dc.contributor.authorLang, Andras
dc.contributor.authorLaurents, Douglas
dc.contributor.authorLecoq, Lauriane
dc.contributor.authorLinhard, Verena
dc.contributor.authorLöhr, Frank
dc.contributor.authorMalki, Anas
dc.contributor.authorBessa, Luiza Mamigonian
dc.contributor.authorMartin, Rachel W
dc.contributor.authorMatzel, Tobias
dc.contributor.authorMaurin, Damien
dc.contributor.authorMcNutt, Seth W
dc.contributor.authorMebus-Antunes, Nathane Cunha
dc.contributor.authorMeier, Beat H
dc.contributor.authorMeiser, Nathalie
dc.contributor.authorMompeán, Miguel
dc.contributor.authorMonaca, Elisa
dc.contributor.authorMontserret, Roland
dc.contributor.authorMariño Perez, Laura
dc.contributor.authorMoser, Celine
dc.contributor.authorMuhle-Goll, Claudia
dc.contributor.authorNeves-Martins, Thais Cristtina
dc.contributor.authorNi, Xiamonin
dc.contributor.authorNorton-Baker, Brenna
dc.contributor.authorPierattelli, Roberta
dc.contributor.authorPontoriero, Letizia
dc.contributor.authorPustovalova, Yulia
dc.contributor.authorOhlenschläger, Oliver
dc.contributor.authorOrts, Julien
dc.contributor.authorDa Poian, Andrea T
dc.contributor.authorPyper, Dennis J
dc.contributor.authorRichter, Christian
dc.contributor.authorRiek, Roland
dc.contributor.authorRienstra, Chad M
dc.contributor.authorRobertson, Angus
dc.contributor.authorPinheiro, Anderson S
dc.contributor.authorSabbatella, Raffaele
dc.contributor.authorSalvi, Nicola
dc.contributor.authorSaxena, Krishna
dc.contributor.authorSchulte, Linda
dc.contributor.authorSchiavina, Marco
dc.contributor.authorSchwalbe, Harald
dc.contributor.authorSilber, Mara
dc.contributor.authorAlmeida, Marcius da Silva
dc.contributor.authorSprague-Piercy, Marc A
dc.contributor.authorSpyroulias, Georgios A
dc.contributor.authorSreeramulu, Sridhar
dc.contributor.authorTants, Jan-Niklas
dc.contributor.authorTārs, Kaspars
dc.contributor.authorTorres, Felix
dc.contributor.authorTöws, Sabrina
dc.contributor.authorTreviño, Miguel Á
dc.contributor.authorTrucks, Sven
dc.contributor.authorTsika, Aikaterini C
dc.contributor.authorVarga, Krisztina
dc.contributor.authorWang, Ying
dc.contributor.authorWeber, Marco E
dc.contributor.authorWeigand, Julia E
dc.contributor.authorWiedemann, Christoph
dc.contributor.authorWirmer-Bartoschek, Julia
dc.contributor.authorWirtz Martin, Maria Alexandra
dc.contributor.authorZehnder, Johannes
dc.contributor.authorHengesbach, Martin
dc.contributor.authorSchlundt, Andreas
dc.date.accessioned2021-07-13T14:17:13Z
dc.date.available2021-07-13T14:17:13Z
dc.date.issued2021-05-10
dc.identifier.citationront Mol Biosci. 2021 May 10;8:653148. doi: 10.3389/fmolb.2021.653148.en_US
dc.identifier.issn2296-889X
dc.identifier.pmid34041264
dc.identifier.doi10.3389/fmolb.2021.653148
dc.identifier.urihttp://hdl.handle.net/10033/622936
dc.description.abstractThe highly infectious disease COVID-19 caused by the Betacoronavirus SARS-CoV-2 poses a severe threat to humanity and demands the redirection of scientific efforts and criteria to organized research projects. The international COVID19-NMR consortium seeks to provide such new approaches by gathering scientific expertise worldwide. In particular, making available viral proteins and RNAs will pave the way to understanding the SARS-CoV-2 molecular components in detail. The research in COVID19-NMR and the resources provided through the consortium are fully disclosed to accelerate access and exploitation. NMR investigations of the viral molecular components are designated to provide the essential basis for further work, including macromolecular interaction studies and high-throughput drug screening. Here, we present the extensive catalog of a holistic SARS-CoV-2 protein preparation approach based on the consortium's collective efforts. We provide protocols for the large-scale production of more than 80% of all SARS-CoV-2 proteins or essential parts of them. Several of the proteins were produced in more than one laboratory, demonstrating the high interoperability between NMR groups worldwide. For the majority of proteins, we can produce isotope-labeled samples of HSQC-grade. Together with several NMR chemical shift assignments made publicly available on covid19-nmr.com, we here provide highly valuable resources for the production of SARS-CoV-2 proteins in isotope-labeled form.en_US
dc.language.isoenen_US
dc.publisherFrontiersen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectCOVID-19en_US
dc.subjectNMR spectroscopyen_US
dc.subjectSARS-CoV-2en_US
dc.subjectaccessory proteinsen_US
dc.subjectcell-free protein synthesisen_US
dc.subjectintrinsically disordered regionen_US
dc.subjectnonstructural proteinsen_US
dc.subjectstructural proteinsen_US
dc.titleLarge-Scale Recombinant Production of the SARS-CoV-2 Proteome for High-Throughput and Structural Biology Applications.en_US
dc.typeArticleen_US
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalFrontiers in molecular biosciencesen_US
dc.source.volume8
dc.source.beginpage653148
dc.source.endpage
refterms.dateFOA2021-07-13T14:17:14Z
dc.source.journaltitleFrontiers in molecular biosciences
dc.source.countrySwitzerland


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International