Significant compartment-specific impact of different RNA extraction methods and PCR assays on the sensitivity of hepatitis E virus detection.

Behrendt, Patrick; Bremer, Birgit; Todt, Daniel; Steinmann, Eike; Manns, Michael Peter; Cornberg, Markus; Wedemeyer, Heiner; Maasoumy, Benjamin

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Authors

Behrendt, Patrick
Bremer, Birgit
Todt, Daniel
Steinmann, Eike
Manns, Michael Peter
Cornberg, Markus

Wedemeyer, Heiner
Maasoumy, Benjamin

Issue Date

2021-03-22

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Abstract

We determined the limit of detection of the RealStar HEV RT-PCR V2.0 Kit (altona Diagnostics, RS) utilizing 3 RNA extraction methods (COBAS® AmpliPrep Total Nucleic Acid Isolation Kit, TNAi Roche; MagNA Pure 96 DNA, Viral NA SV Kit, MgP; QIAamp Viral RNA mini Kit Qiagen; VRK) in plasma and stool. The most sensitive method was evaluated in a total of 307 longitudinal samples of patients with HEV infection (acute = 18/chronic = 36) and compared to results with the former diagnostic standard of our centre (TNAi/FastTrack Diagnostic; FTD).

Citation

Liver Int. 2021 Aug;41(8):1815-1823. doi: 10.1111/liv.14870. Epub 2021 Mar 22.

Affiliation

CiiM, Zentrum für individualisierte Infektionsmedizin, Feodor-Lynen-Str.7, 30625 Hannover.

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Article

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EISSN

1478-3231

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publications of the working group of computational biology for individualized medicine ([CiiM] BIIM)

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Except where otherwise noted, this item's license is described as Attribution 4.0 International