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dc.contributor.authorBehrendt, Patrick
dc.contributor.authorBremer, Birgit
dc.contributor.authorTodt, Daniel
dc.contributor.authorSteinmann, Eike
dc.contributor.authorManns, Michael Peter
dc.contributor.authorCornberg, Markus
dc.contributor.authorWedemeyer, Heiner
dc.contributor.authorMaasoumy, Benjamin
dc.date.accessioned2021-07-27T12:03:35Z
dc.date.available2021-07-27T12:03:35Z
dc.date.issued2021-03-22
dc.identifier.citationLiver Int. 2021 Aug;41(8):1815-1823. doi: 10.1111/liv.14870. Epub 2021 Mar 22.en_US
dc.identifier.pmid33683813
dc.identifier.doi10.1111/liv.14870
dc.identifier.urihttp://hdl.handle.net/10033/622961
dc.description.abstractWe determined the limit of detection of the RealStar HEV RT-PCR V2.0 Kit (altona Diagnostics, RS) utilizing 3 RNA extraction methods (COBAS® AmpliPrep Total Nucleic Acid Isolation Kit, TNAi Roche; MagNA Pure 96 DNA, Viral NA SV Kit, MgP; QIAamp Viral RNA mini Kit Qiagen; VRK) in plasma and stool. The most sensitive method was evaluated in a total of 307 longitudinal samples of patients with HEV infection (acute = 18/chronic = 36) and compared to results with the former diagnostic standard of our centre (TNAi/FastTrack Diagnostic; FTD).en_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectHEV RNA assayen_US
dc.subjectHEV infectionen_US
dc.subjectHEV treatmenten_US
dc.subjectresponse-guided therapyen_US
dc.subjectribavirinen_US
dc.subjectviral RNA extractionen_US
dc.titleSignificant compartment-specific impact of different RNA extraction methods and PCR assays on the sensitivity of hepatitis E virus detection.en_US
dc.typeArticleen_US
dc.identifier.eissn1478-3231
dc.contributor.departmentCiiM, Zentrum für individualisierte Infektionsmedizin, Feodor-Lynen-Str.7, 30625 Hannover.en_US
dc.identifier.journalLiver international : official journal of the International Association for the Study of the Liveren_US
dc.source.volume41
dc.source.issue8
dc.source.beginpage1815
dc.source.endpage1823
refterms.dateFOA2021-07-27T12:03:36Z
dc.source.journaltitleLiver international : official journal of the International Association for the Study of the Liver
dc.source.countryUnited States


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International