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dc.contributor.authorTaxiarchi, Chrysanthi
dc.contributor.authorBeaghton, Andrea
dc.contributor.authorDon, Nayomi Illansinhage
dc.contributor.authorKyrou, Kyros
dc.contributor.authorGribble, Matthew
dc.contributor.authorShittu, Dammy
dc.contributor.authorCollins, Scott P
dc.contributor.authorBeisel, Chase L
dc.contributor.authorGalizi, Roberto
dc.contributor.authorCrisanti, Andrea
dc.date.accessioned2021-07-29T11:59:03Z
dc.date.available2021-07-29T11:59:03Z
dc.date.issued2021-06-25
dc.identifier.citationNat Commun. 2021 Jun 25;12(1):3977. doi: 10.1038/s41467-021-24214-5.en_US
dc.identifier.pmid34172748
dc.identifier.doi10.1038/s41467-021-24214-5
dc.identifier.urihttp://hdl.handle.net/10033/622967
dc.description.abstractCRISPR-based gene drives offer promising means to reduce the burden of pests and vector-borne diseases. These techniques consist of releasing genetically modified organisms carrying CRISPR-Cas nucleases designed to bias their inheritance and rapidly propagate desired modifications. Gene drives can be intended to reduce reproductive capacity of harmful insects or spread anti-pathogen effectors through wild populations, even when these confer fitness disadvantages. Technologies capable of halting the spread of gene drives may prove highly valuable in controlling, counteracting, and even reverting their effect on individual organisms as well as entire populations. Here we show engineering and testing of a genetic approach, based on the germline expression of a phage-derived anti-CRISPR protein (AcrIIA4), able to inactivate CRISPR-based gene drives and restore their inheritance to Mendelian rates in the malaria vector Anopheles gambiae. Modeling predictions and cage testing show that a single release of male mosquitoes carrying the AcrIIA4 protein can block the spread of a highly effective suppressive gene drive preventing population collapse of caged malaria mosquitoes.en_US
dc.language.isoenen_US
dc.publisherNature researchen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleA genetically encoded anti-CRISPR protein constrains gene drive spread and prevents population suppression.en_US
dc.typeArticleen_US
dc.identifier.eissn2041-1723
dc.contributor.departmentHIRI, Helmholtz-Institut für RNA-basierte Infektionsforschung, Josef-Shneider Strasse 2, 97080 Würzburg, Germany.en_US
dc.identifier.journalNature communicationsen_US
dc.source.volume12
dc.source.issue1
dc.source.beginpage3977
dc.source.endpage
refterms.dateFOA2021-07-29T11:59:04Z
dc.source.journaltitleNature communications
dc.source.countryEngland


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International