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dc.contributor.authorStierhof, Marc
dc.contributor.authorMyronovskyi, Maksym
dc.contributor.authorZapp, Josef
dc.contributor.authorLuzhetskyy, Andriy N
dc.date.accessioned2021-07-29T14:12:32Z
dc.date.available2021-07-29T14:12:32Z
dc.date.issued2021-06-28
dc.identifier.citationMicroorganisms. 2021 Jun 28;9(7):1396. doi: 10.3390/microorganisms9071396.en_US
dc.identifier.issn2076-2607
dc.identifier.pmid34203385
dc.identifier.doi10.3390/microorganisms9071396
dc.identifier.urihttp://hdl.handle.net/10033/622970
dc.description.abstractStreptomyces are producers of valuable secondary metabolites with unique scaffolds that perform a plethora of biological functions. Nonribosomal peptides are of special interest due to their variety and complexity. They are synthesized by nonribosomal peptide synthetases, large biosynthetic machineries that are encoded in the genome of many Streptomyces species. The identification of new peptides and the corresponding biosynthetic gene clusters is of major interest since knowledge can be used to facilitate combinatorial biosynthesis and chemical semisynthesis of natural products. The recently discovered bosamycins are linear octapeptides with an interesting 5-OMe tyrosine moiety and various modifications at the N-terminus. In this study, the new cyclic depsibosamycins B, C, and D from Streptomyces aurantiacus LU19075 were discovered. In comparison to the linear bosamycins B, C, and D, which were also produced by the strain, the cyclic depsibosamycins showed a side-chain-to-tail lactonization of serine and glycine, leading to a ring of four amino acids. In silico identification and heterologous expression of the depsibosamycin (dbm) gene cluster indicated that the cyclic peptides, rather than the linear derivatives, are the main products of the cluster.en_US
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectNRPSen_US
dc.subjectStreptomycesen_US
dc.subjectbosamycinen_US
dc.subjectcyclic peptideen_US
dc.subjectheterologous expressionen_US
dc.titleDiscovery and Heterologous Production of New Cyclic Depsibosamycins.en_US
dc.typeArticleen_US
dc.contributor.departmentHIPS, Helmholtz-Institut für Pharmazeutische Forschung Saarland, Universitätscampus E8.1 66123 Saarbrücken, Germany.en_US
dc.identifier.journalMicroorganismsen_US
dc.source.volume9
dc.source.issue7
refterms.dateFOA2021-07-29T14:12:32Z
dc.source.journaltitleMicroorganisms
dc.source.countrySwitzerland


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International