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dc.contributor.authorHoang, Quynh Trang Mi
dc.contributor.authorNguyen, Van Kinh
dc.contributor.authorOberacher, Herbert
dc.contributor.authorFuchs, Dietmar
dc.contributor.authorHernandez-Vargas, Esteban A
dc.contributor.authorBorucki, Katrin
dc.contributor.authorWaldburg, Nadine
dc.contributor.authorWippermann, Jens
dc.contributor.authorSchreiber, Jens
dc.contributor.authorBruder, Dunja
dc.contributor.authorVeluswamy, Priya
dc.date.accessioned2021-07-29T14:31:42Z
dc.date.available2021-07-29T14:31:42Z
dc.date.issued2021-06-08
dc.identifier.citationFront Med (Lausanne). 2021 Jun 8;8:676058. doi: 10.3389/fmed.2021.676058.en_US
dc.identifier.issn2296-858X
dc.identifier.pmid34169084
dc.identifier.doi10.3389/fmed.2021.676058
dc.identifier.urihttp://hdl.handle.net/10033/622971
dc.description.abstractCOPD and asthma are two distinct but sometimes overlapping diseases exhibiting varying degrees and types of inflammation on different stages of the disease. Although several biomarkers are defined to estimate the inflammatory endotype and stages in these diseases, there is still a need for new markers and potential therapeutic targets. We investigated the levels of a phytohormone, abscisic acid (ABA) and its receptor, LANCL2, in COPD patients and asthmatics. In addition, PPAR-γ that is activated by ABA in a ligand-binding domain-independent manner was also included in the study. In this study, we correlated ABA with COPD-propagating factors to define the possible role of ABA, in terms of immune regulation, inflammation, and disease stages. We collected blood from 101 COPD patients, 52 asthmatics, and 57 controls. Bronchoscopy was performed on five COPD patients and 29 controls. We employed (i) liquid chromatography-tandem mass spectrometry and HPLC to determine the ABA and indoleamine 2,3-dioxygenase levels, respectively; (ii) real-time PCR to quantify the gene expression of LANCL2 and PPAR-γ; (iii) Flow cytometry to quantify adipocytokines; and (iv) immunoturbidimetry and ELISA to measure CRP and cytokines, respectively. Finally, a multinomial regression model was used to predict the probability of using ABA as a biomarker. Blood ABA levels were significantly reduced in COPD patients and asthmatics compared to age- and gender-matched normal controls. However, PPAR-γ was elevated in COPD patients. Intriguingly, ABA was positively correlated with immune-regulatory factors and was negatively correlated with inflammatory markers, in COPD. Of note, ABA was increased in advanced COPD stages. We thereby conclude that ABA might be involved in regulation of COPD pathogenesis and might be regarded as a potential biomarker for COPD stages.en_US
dc.language.isoenen_US
dc.publisherFrontiersen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectCOPDen_US
dc.subjectLanCL2en_US
dc.subjectPPAR-γen_US
dc.subjectabscisic aciden_US
dc.subjectasthmaen_US
dc.titleSerum Concentration of the Phytohormone Abscisic Acid Is Associated With Immune-Regulatory Mediators and Is a Potential Biomarker of Disease Severity in Chronic Obstructive Pulmonary Disease.en_US
dc.typeArticleen_US
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalFrontiers in medicineen_US
dc.source.volume8
dc.source.beginpage676058
dc.source.endpage
refterms.dateFOA2021-07-29T14:31:42Z
dc.source.journaltitleFrontiers in medicine
dc.source.countrySwitzerland


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International