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dc.contributor.authorWalch, Philipp
dc.contributor.authorSelkrig, Joel
dc.contributor.authorKnodler, Leigh A
dc.contributor.authorRettel, Mandy
dc.contributor.authorStein, Frank
dc.contributor.authorFernandez, Keith
dc.contributor.authorViéitez, Cristina
dc.contributor.authorPotel, Clément M
dc.contributor.authorScholzen, Karoline
dc.contributor.authorGeyer, Matthias
dc.contributor.authorRottner, Klemens
dc.contributor.authorSteele-Mortimer, Olivia
dc.contributor.authorSavitski, Mikhail M
dc.contributor.authorHolden, David W
dc.contributor.authorTypas, Athanasios
dc.date.accessioned2021-08-04T13:57:20Z
dc.date.available2021-08-04T13:57:20Z
dc.date.issued2021-07-02
dc.identifier.citationl Host Microbe. 2021 Jul 2:S1931-3128(21)00282-1. doi: 10.1016/j.chom.2021.06.004. Epub ahead of print.en_US
dc.identifier.pmid34237247
dc.identifier.doi10.1016/j.chom.2021.06.004
dc.identifier.urihttp://hdl.handle.net/10033/622977
dc.description.abstractIntracellular bacterial pathogens inject effector proteins to hijack host cellular processes and promote their survival and proliferation. To systematically map effector-host protein-protein interactions (PPIs) during infection, we generated a library of 32 Salmonella enterica serovar Typhimurium (STm) strains expressing chromosomally encoded affinity-tagged effectors and quantified PPIs in macrophages and epithelial cells. We identified 446 effector-host PPIs, 25 of which were previously described, and validated 13 by reciprocal co-immunoprecipitation. While effectors converged on the same host cellular processes, most had multiple targets, which often differed between cell types. We demonstrate that SseJ, SseL, and SifA modulate cholesterol accumulation at the Salmonella-containing vacuole (SCV) partially via the cholesterol transporter Niemann-Pick C1 protein. PipB recruits the organelle contact site protein PDZD8 to the SCV, and SteC promotes actin bundling by phosphorylating formin-like proteins. This study provides a method for probing host-pathogen PPIs during infection and a resource for interrogating STm effector mechanisms.en_US
dc.language.isoenen_US
dc.publisherCell Pressen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectFMNLen_US
dc.subjectNPC1en_US
dc.subjectPDZD8en_US
dc.subjectactin bundlingen_US
dc.subjectbacterial pathogenen_US
dc.subjectcholesterol traffickingen_US
dc.subjecteffectorsen_US
dc.subjectprotein-protein interactionsen_US
dc.titleGlobal mapping ofSalmonella entericahost protein-protein interactions during infection.en_US
dc.typeArticleen_US
dc.identifier.eissn1934-6069
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalCell host & microbeen_US
refterms.dateFOA2021-08-04T13:57:20Z
dc.source.journaltitleCell host & microbe
dc.source.countryUnited States


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International