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dc.contributor.authorWami, Haleluya
dc.contributor.authorWallenstein, Alexander
dc.contributor.authorSauer, Daniel
dc.contributor.authorStoll, Monika
dc.contributor.authorvon Bünau, Rudolf
dc.contributor.authorOswald, Eric
dc.contributor.authorMüller, Rolf
dc.contributor.authorDobrindt, Ulrich
dc.date.accessioned2021-08-05T09:49:05Z
dc.date.available2021-08-05T09:49:05Z
dc.date.issued2021-06-15
dc.identifier.citationMicrob Genom. 2021 Jun;7(6). doi: 10.1099/mgen.0.000577.en_US
dc.identifier.pmid34128785
dc.identifier.doi10.1099/mgen.0.000577
dc.identifier.urihttp://hdl.handle.net/10033/622978
dc.description.abstractThe bacterial genotoxin colibactin interferes with the eukaryotic cell cycle by causing dsDNA breaks. It has been linked to bacterially induced colorectal cancer in humans. Colibactin is encoded by a 54 kb genomic region in Enterobacteriaceae. The colibactin genes commonly co-occur with the yersiniabactin biosynthetic determinant. Investigating the prevalence and sequence diversity of the colibactin determinant and its linkage to the yersiniabactin operon in prokaryotic genomes, we discovered mainly species-specific lineages of the colibactin determinant and classified three main structural settings of the colibactin-yersiniabactin genomic region in Enterobacteriaceae. The colibactin gene cluster has a similar but not identical evolutionary track to that of the yersiniabactin operon. Both determinants could have been acquired on several occasions and/or exchanged independently between enterobacteria by horizontal gene transfer. Integrative and conjugative elements play(ed) a central role in the evolution and structural diversity of the colibactin-yersiniabactin genomic region. Addition of an activating and regulating module (clbAR) to the biosynthesis and transport module (clbB-S) represents the most recent step in the evolution of the colibactin determinant. In a first attempt to correlate colibactin expression with individual lineages of colibactin determinants and different bacterial genetic backgrounds, we compared colibactin expression of selected enterobacterial isolates in vitro. Colibactin production in the tested Klebsiella species and Citrobacter koseri strains was more homogeneous and generally higher than that in most of the Escherichia coli isolates studied. Our results improve the understanding of the diversity of colibactin determinants and its expression level, and may contribute to risk assessment of colibactin-producing enterobacteria.en_US
dc.language.isoenen_US
dc.publisherMicrobiology Societyen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectCitrobacteren_US
dc.subjectEscherichia colien_US
dc.subjectKlebsiellaen_US
dc.subjectcytopathic effecten_US
dc.subjecthigh pathogenicity islanden_US
dc.subjectpolyketideen_US
dc.subjectsecondary metaboliteen_US
dc.titleInsights into evolution and coexistence of the colibactin- and yersiniabactin secondary metabolite determinants in enterobacterial populations.en_US
dc.typeArticleen_US
dc.identifier.eissn2057-5858
dc.contributor.departmentHIPS, Helmholtz-Institut für Pharmazeutische Forschung Saarland, Universitätscampus E8.1 66123 Saarbrücken, Germany.en_US
dc.identifier.journalMicrobial genomicsen_US
dc.source.volume7
dc.source.issue6
refterms.dateFOA2021-08-05T09:49:06Z
dc.source.journaltitleMicrobial genomics
dc.source.countryEngland


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Attribution 4.0 International
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