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dc.contributor.authorFiorini, Federica
dc.contributor.authorBorgonuovo, Camilla
dc.contributor.authorFerrante, Maria Immacolata
dc.contributor.authorBrönstrup, Mark
dc.date.accessioned2021-08-06T12:45:36Z
dc.date.available2021-08-06T12:45:36Z
dc.date.issued2020-06-14
dc.identifier.citationMar Drugs. 2020 Jun 14;18(6):313. doi: 10.3390/md18060313.en_US
dc.identifier.pmid32545923
dc.identifier.doi10.3390/md18060313
dc.identifier.urihttp://hdl.handle.net/10033/622984
dc.description.abstractPseudo-nitzschia multistriata is a planktonic marine diatom with a diplontic life cycle comprising a short sexual phase, during which gametes are produced following the encounter of two diploid cells of opposite mating type (MT). Gene expression studies have highlighted the presence of substantial changes occurring at the onset of sexual reproduction. Herein, we have hypothesized that the amount and nature of cellular metabolites varies along the mating process. To capture the metabolome of Pseudo-nitzschia multistriata at different harvesting times in an unbiased manner, we undertook an untargeted metabolomics approach based on liquid chromatography-tandem mass spectrometry. Using three different extraction steps, the method revealed pronounced differences in the metabolic profiles between control cells in the vegetative phase (MT+ and MT-) and mixed strains of opposite MTs (cross) undergoing sexual reproduction. Of the 2408 high-quality features obtained, 70 known metabolites could be identified based on in-house libraries and online databases; additional 46 features could be classified by molecular networking of tandem mass spectra. The reduction of phytol detected in the cross can be linked to the general downregulation of photosynthesis during sexual reproduction observed elsewhere. Moreover, the role of highly regulated compounds such as 7-dehydrodesmosterol, whose changes in abundance were the highest in the experiment, oleamide, ectoine, or trigonelline is discussed.en_US
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectPseudo-nitzschia multistriataen_US
dc.subjectdiatomsen_US
dc.subjectmarine natural productsen_US
dc.subjectmass spectrometryen_US
dc.subjectmetabolomicsen_US
dc.subjectsexual reproductionen_US
dc.titleA Metabolomics Exploration of the Sexual Phase in the Marine Diatom Pseudo-nitzschia multistriata.en_US
dc.typeArticleen_US
dc.identifier.eissn1660-3397
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalMarine drugsen_US
dc.source.volume18
dc.source.issue6
refterms.dateFOA2021-08-06T12:45:37Z
dc.source.journaltitleMarine drugs
dc.source.countrySwitzerland


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International