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dc.contributor.authorKopfnagel, Verena
dc.contributor.authorDreyer, Sylvia
dc.contributor.authorZeitvogel, Jana
dc.contributor.authorPieper, Dietmar H
dc.contributor.authorBuch, Anna
dc.contributor.authorSodeik, Beate
dc.contributor.authorRademacher, Franziska
dc.contributor.authorHarder, Jürgen
dc.date.accessioned2021-08-16T14:21:59Z
dc.date.available2021-08-16T14:21:59Z
dc.date.issued2021-06-27
dc.identifier.citationAllergy. 2021 Jun 27. doi: 10.1111/all.14992. Epub ahead of print.en_US
dc.identifier.pmid34176149
dc.identifier.doi10.1111/all.14992
dc.identifier.urihttp://hdl.handle.net/10033/622990
dc.description.abstractBackground: The high susceptibility of AD patients to microbial skin infections has been attributed to a deficient antimicrobial peptide (AMP) expression, which is contradicted by a growing amount of recent studies clearly demonstrating that AMP expression is not impaired in lesional skin of AD patients. The reasons for the high susceptibility of AD patients to microbial infections are still unknown. Methods: The influence of self-DNA on the antimicrobial activity of RNase 7, LL-37, and hBD2 has been investigated using antibacterial and antiviral assays. The amount of self-DNA on skin has been analyzed by skin rinsings and subsequent quantification using dsDNA assays. DNA source was identified by qPCR. Results: Complex formation of the AMPs with self-DNA significantly impaired their antibacterial activity against Staphylococcus aureus and their antiviral activity against HSV-1. The inhibition of the antibacterial activity was dependent on the DNA concentration but not on the length of the DNA molecules. Of note, we detected significant higher amounts of cell-free self-DNA in skin rinses taken from lesional AD skin compared to skin rinses from non-lesional skin and from normal skin of healthy donors. Consequently, rinse solution from AD lesional skin prevented antibacterial activity of LL-37. Conclusion: Our study indicates that extracellular self-DNA is released in considerable amounts in AD skin lesions and AMP-self-DNA-complex formation leads to a significant loss of antibacterial and antiviral activity in atopic dermatitis. Studies on strategies to reduce the amount of extracellular DNA in AD are needed to identify possible methods relevant in clinical settings.en_US
dc.language.isoenen_US
dc.publisherJohn Wiley & Sons LTD.en_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectHSV-1en_US
dc.subjectStaphylococcus aureusen_US
dc.subjectDNAen_US
dc.subjectantimicrobial peptidesen_US
dc.subjectatopic dermatitisen_US
dc.titleFree human DNA attenuates the activity of antimicrobial peptides in atopic dermatitis.en_US
dc.typeArticleen_US
dc.identifier.eissn1398-9995
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalAllergyen_US
refterms.dateFOA2021-08-16T14:21:59Z
dc.source.journaltitleAllergy
dc.source.countryDenmark


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International