Show simple item record

dc.contributor.authorKnorr, Jakob
dc.contributor.authorSharafutdinov, Irshad
dc.contributor.authorFiedler, Florian
dc.contributor.authorSoltan Esmaeili, Delara
dc.contributor.authorRohde, Manfred
dc.contributor.authorRottner, Klemens
dc.contributor.authorBackert, Steffen
dc.contributor.authorTegtmeyer, Nicole
dc.date.accessioned2021-08-25T13:59:11Z
dc.date.available2021-08-25T13:59:11Z
dc.date.issued2021-06-03
dc.identifier.citationInt J Mol Sci. 2021 Jun 3;22(11):6045. doi: 10.3390/ijms22116045.en_US
dc.identifier.pmid34205064
dc.identifier.doi10.3390/ijms22116045
dc.identifier.urihttp://hdl.handle.net/10033/623002
dc.description.abstractCortactin is a well-known regulatory protein of the host actin cytoskeleton and represents an attractive target of microbial pathogens like Helicobacter pylori. H. pylori manipulates cortactin's phosphorylation status by type-IV secretion-dependent injection of its virulence protein CagA. Multiple host tyrosine kinases, like FAK, Src, and Abl, are activated during infection, but the pathway(s) involved is (are) not yet fully established. Among them, Src and Abl target CagA and stimulate tyrosine phosphorylation of the latter at its EPIYA-motifs. To investigate the role of cortactin in more detail, we generated a CRISPR/Cas9 knockout of cortactin in AGS gastric epithelial cells. Surprisingly, we found that FAK, Src, and Abl kinase activities were dramatically downregulated associated with widely diminished CagA phosphorylation in cortactin knockout cells compared to the parental control. Together, we report here a yet unrecognized cortactin-dependent signaling pathway involving FAK, Src, and Abl activation, and controlling efficient phosphorylation of injected CagA during infection. Thus, the cortactin status could serve as a potential new biomarker of gastric cancer development.en_US
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectAblen_US
dc.subjectFAKen_US
dc.subjectHelicobacteren_US
dc.subjectSrcen_US
dc.subjectcanceren_US
dc.subjectcortactinen_US
dc.subjectpathogenesisen_US
dc.subjectpathogenicity islanden_US
dc.subjectsignalingen_US
dc.subjectvirulenceen_US
dc.titleCortactin Is Required for Efficient FAK, Src and Abl Tyrosine Kinase Activation and Phosphorylation of CagA.en_US
dc.typeArticleen_US
dc.identifier.eissn1422-0067
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalInternational journal of molecular sciencesen_US
dc.source.volume22
dc.source.issue11
refterms.dateFOA2021-08-25T13:59:12Z
dc.source.journaltitleInternational journal of molecular sciences
dc.source.countrySwitzerland


Files in this item

Thumbnail
Name:
Knorr et al.pdf
Size:
5.077Mb
Format:
PDF
Description:
Open Access publication

This item appears in the following Collection(s)

Show simple item record

Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International