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dc.contributor.authorHorbal, Liliya
dc.contributor.authorStierhof, Marc
dc.contributor.authorPalusczak, Anja
dc.contributor.authorEckert, Nikolas
dc.contributor.authorZapp, Josef
dc.contributor.authorLuzhetskyy, Andriy N
dc.date.accessioned2021-08-30T12:26:53Z
dc.date.available2021-08-30T12:26:53Z
dc.date.issued2021-07-28
dc.identifier.citationMicroorganisms. 2021 Jul 28;9(8):1609. doi: 10.3390/microorganisms9081609.en_US
dc.identifier.issn2076-2607
dc.identifier.pmid34442689
dc.identifier.doi10.3390/microorganisms9081609
dc.identifier.urihttp://hdl.handle.net/10033/623006
dc.description.abstractTargeted genome mining is an efficient method of biosynthetic gene cluster prioritization within constantly growing genome databases. Using two capreomycidine biosynthesis genes, alpha-ketoglutarate-dependent arginine beta-hydroxylase and pyridoxal-phosphate-dependent aminotransferase, we identified two types of clusters: one type containing both genes involved in the biosynthesis of the abovementioned moiety, and other clusters including only arginine hydroxylase. Detailed analysis of one of the clusters, the flk cluster from Streptomyces albus, led to the identification of a cyclic peptide that contains a rare D-capreomycidine moiety for the first time. The absence of the pyridoxal-phosphate-dependent aminotransferase gene in the flk cluster is compensated by the XNR_1347 gene in the S. albus genome, whose product is responsible for biosynthesis of the abovementioned nonproteinogenic amino acid. Herein, we report the structure of cyclofaulknamycin and the characteristics of its biosynthetic gene cluster, biosynthesis and bioactivity profile.en_US
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectD-capreomycidineen_US
dc.subjectStreptomycesen_US
dc.subjectcyclofaulknamycinen_US
dc.subjectcyclopeptideen_US
dc.titleCyclofaulknamycin with the Rare Amino Acid D-capreomycidine Isolated from a Well-Characterized Strain.en_US
dc.typeArticleen_US
dc.contributor.departmentHIPS, Helmholtz-Institut für Pharmazeutische Forschung Saarland, Universitätscampus E8.1 66123 Saarbrücken, Germany.en_US
dc.identifier.journalMicroorganismsen_US
dc.source.volume9
dc.source.issue8
refterms.dateFOA2021-08-30T12:26:54Z
dc.source.journaltitleMicroorganisms
dc.source.countrySwitzerland


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International