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dc.contributor.authorLiu, Jiaqi
dc.contributor.authorZhou, Haibo
dc.contributor.authorYang, Zhiyu
dc.contributor.authorWang, Xue
dc.contributor.authorChen, Hanna
dc.contributor.authorZhong, Lin
dc.contributor.authorZheng, Wentao
dc.contributor.authorNiu, Weijing
dc.contributor.authorWang, Sen
dc.contributor.authorRen, Xiangmei
dc.contributor.authorZhong, Guannan
dc.contributor.authorWang, Yan
dc.contributor.authorDing, Xiaoming
dc.contributor.authorMüller, Rolf
dc.contributor.authorZhang, Youming
dc.contributor.authorBian, Xiaoying
dc.date.accessioned2021-09-08T13:10:55Z
dc.date.available2021-09-08T13:10:55Z
dc.date.issued2021-07-23
dc.identifier.citationNat Commun. 2021 Jul 23;12(1):4347. doi: 10.1038/s41467-021-24645-0.en_US
dc.identifier.pmid34301933
dc.identifier.doi10.1038/s41467-021-24645-0
dc.identifier.urihttp://hdl.handle.net/10033/623018
dc.description.abstractHeterologous expression of biosynthetic gene clusters (BGCs) avails yield improvements and mining of natural products, but it is limited by lacking of more efficient Gram-negative chassis. The proteobacterium Schlegelella brevitalea DSM 7029 exhibits potential for heterologous BGC expression, but its cells undergo early autolysis, hindering further applications. Herein, we rationally construct DC and DT series genome-reduced S. brevitalea mutants by sequential deletions of endogenous BGCs and the nonessential genomic regions, respectively. The DC5 to DC7 mutants affect growth, while the DT series mutants show improved growth characteristics with alleviated cell autolysis. The yield improvements of six proteobacterial natural products and successful identification of chitinimides from Chitinimonas koreensis via heterologous expression in DT mutants demonstrate their superiority to wild-type DSM 7029 and two commonly used Gram-negative chassis Escherichia coli and Pseudomonas putida. Our study expands the panel of Gram-negative chassis and facilitates the discovery of natural products by heterologous expression.en_US
dc.language.isoenen_US
dc.publisherNature publishing groupen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleRational construction of genome-reduced Burkholderiales chassis facilitates efficient heterologous production of natural products from proteobacteria.en_US
dc.typeArticleen_US
dc.identifier.eissn2041-1723
dc.contributor.departmentHIPS, Helmholtz-Institut für Pharmazeutische Forschung Saarland, Universitätscampus E8.1 66123 Saarbrücken, Germany.en_US
dc.identifier.journalNature communicationsen_US
dc.source.volume12
dc.source.issue1
dc.source.beginpage4347
dc.source.endpage
refterms.dateFOA2021-09-08T13:10:56Z
dc.source.journaltitleNature communications
dc.source.countryEngland


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International