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dc.contributor.authorHorstmann, Justus C
dc.contributor.authorThorn, Chelsea R
dc.contributor.authorCarius, Patrick
dc.contributor.authorGraef, Florian
dc.contributor.authorMurgia, Xabier
dc.contributor.authorde Souza Carvalho-Wodarz, Cristiane
dc.contributor.authorLehr, Claus-Michael
dc.date.accessioned2021-09-27T12:39:19Z
dc.date.available2021-09-27T12:39:19Z
dc.date.issued2021-04-21
dc.identifier.citationFront Bioeng Biotechnol. 2021 Apr 21;9:643491. doi: 10.3389/fbioe.2021.643491.en_US
dc.identifier.issn2296-4185
dc.identifier.pmid33968912
dc.identifier.doi10.3389/fbioe.2021.643491
dc.identifier.urihttp://hdl.handle.net/10033/623046
dc.description.abstractThe deposition of pre-metered doses (i.e., defined before and not after exposition) at the air-liquid interface of viable pulmonary epithelial cells remains an important but challenging task for developing aerosol medicines. While some devices allow quantification of the deposited dose after or during the experiment, e.g., gravimetrically, there is still no generally accepted way to deposit small pre-metered doses of aerosolized drugs or pharmaceutical formulations, e.g., nanomedicines. Here, we describe a straightforward custom-made device, allowing connection to commercially available nebulizers with standard cell culture plates. Designed to tightly fit into the approximately 12-mm opening of either a 12-well Transwell® insert or a single 24-well plate, a defined dose of an aerosolized liquid can be directly deposited precisely and reproducibly (4.8% deviation) at the air-liquid interface (ALI) of pulmonary cell cultures. The deposited dose can be controlled by the volume of the nebulized solution, which may vary in a range from 20 to 200 μl. The entire nebulization-deposition maneuver is completed after 30 s and is spatially homogenous. After phosphate-buffered saline (PBS) deposition, the viability and barrier properties transepithelial electrical resistance (TEER) of human bronchial epithelial Calu-3 cells were not negatively affected. Straightforward in manufacture and use, the device enables reproducible deposition of metered doses of aerosolized drugs to study the interactions with pulmonary cell cultures grown at ALI conditions.en_US
dc.language.isoenen_US
dc.publisherFrontiersen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectaerosolen_US
dc.subjectair–liquid interfaceen_US
dc.subjectepithelial cellsen_US
dc.subjectinhalationen_US
dc.subjectpulmonary drug deliveryen_US
dc.titleA Custom-Made Device for Reproducibly Depositing Pre-metered Doses of Nebulized Drugs on Pulmonary Cells .en_US
dc.typeArticleen_US
dc.contributor.departmentHIPS, Helmholtz-Institut für Pharmazeutische Forschung Saarland, Universitätscampus E8.1 66123 Saarbrücken, Germany.en_US
dc.identifier.journalFrontiers in bioengineering and biotechnologyen_US
dc.source.volume9
dc.source.beginpage643491
dc.source.endpage
refterms.dateFOA2021-09-27T12:39:20Z
dc.source.journaltitleFrontiers in bioengineering and biotechnology
dc.source.countrySwitzerland


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International