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dc.contributor.authorTomasch, Jürgen
dc.contributor.authorKoppenhöfer, Sonja
dc.contributor.authorLang, Andrew S
dc.date.accessioned2021-09-28T14:03:17Z
dc.date.available2021-09-28T14:03:17Z
dc.date.issued2021-04-29
dc.identifier.citationFront Microbiol. 2021 Apr 29;12:662907. doi: 10.3389/fmicb.2021.662907.en_US
dc.identifier.issn1664-302X
dc.identifier.pmid33995326
dc.identifier.doi10.3389/fmicb.2021.662907
dc.identifier.urihttp://hdl.handle.net/10033/623049
dc.description.abstractMost bacterial chromosomes are circular, with replication starting at one origin (ori) and proceeding on both replichores toward the terminus (ter). Several studies have shown that the location of genes relative to ori and ter can have profound effects on regulatory networks and physiological processes. The CtrA phosphorelay is a gene regulatory system conserved in most alphaproteobacteria. It was first discovered in Caulobacter crescentus where it controls replication and division into a stalked and a motile cell in coordination with other factors. The locations of the ctrA gene and targets of this response regulator on the chromosome affect their expression through replication-induced DNA hemi-methylation and specific positioning along a CtrA activity gradient in the dividing cell, respectively. Here we asked to what extent the location of CtrA regulatory network genes might be conserved in the alphaproteobacteria. We determined the locations of the CtrA phosphorelay and associated genes in closed genomes with unambiguously identifiable ori from members of five alphaproteobacterial orders. The location of the phosphorelay genes was the least conserved in the Rhodospirillales followed by the Sphingomonadales. In the Rhizobiales a trend toward certain chromosomal positions could be observed. Compared to the other orders, the CtrA phosphorelay genes were conserved closer to ori in the Caulobacterales. In contrast, the genes were highly conserved closer to ter in the Rhodobacterales. Our data suggest selection pressure results in differential positioning of CtrA phosphorelay and associated genes in alphaproteobacteria, particularly in the orders Rhodobacterales, Caulobacterales and Rhizobiales that is worth deeper investigation.en_US
dc.language.isoenen_US
dc.publisherFrontiersen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectCtrA phosphorelayen_US
dc.subjectgene expressionen_US
dc.subjectgenome evolutionen_US
dc.subjectgenome organizationen_US
dc.subjectreplicationen_US
dc.titleConnection Between Chromosomal Location and Function of CtrA Phosphorelay Genes in Alphaproteobacteria.en_US
dc.typeArticleen_US
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalFrontiers in microbiologyen_US
dc.source.volume12
dc.source.beginpage662907
dc.source.endpage
refterms.dateFOA2021-09-28T14:03:18Z
dc.source.journaltitleFrontiers in microbiology
dc.source.countrySwitzerland


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International